Phosphatidylcholine-specific phospholipase C, p53 and ROS in the association of apoptosis and senescence in vascular endothelial cells
Phosphatidylcholine-specific phospholipase C, p53 and ROS in the association of apoptosis and senescence in vascular endothelial cells
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磷脂酰胆碱特异性磷脂酶 C、p53 和 ROS 与血管内皮细胞凋亡和衰老的关系
DOI：
10.1016/j.febslet.2006.08.008
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发表时间：
2006-09-04
期刊：
影响因子：
3.5
通讯作者：
Miao, Junying
中科院分区：
文献类型：
--
作者：
Cheng, Yizhe;Zhao, Qitao;Miao, Junying
以前，我们发现磷脂酰胆碱特异性磷脂酶C（PC-PLC）参与了血管内皮细胞（VECs）的凋亡信号转导。在这里，探讨是否PC-PLC参与了血管内皮细胞凋亡和衰老的关联，我们分析了p53的表达和细胞内活性氧（ROS）水平在年轻和衰老的血管内皮细胞抑制PC-PLC活性之前和之后。结果表明，抑制PC-PLC抑制年轻细胞的凋亡和凋亡诱导的p53表达的升高，但在衰老细胞中没有，抑制PC-PLC降低细胞内ROS水平在年轻和衰老细胞。提示PC-PLC参与细胞凋亡与衰老的关系。其功能可能与p53在血管内皮细胞中的表达密切相关。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Previously, we found that phosphatidylcholine-specific phospholipase C (PC-PLC) participated in apoptosis signaling of vascular endothelial cells (VECs). Here, to explore whether PC-PLC is involved in the association of apoptosis and senescence in VECs, we analyzed p53 expression and intracellular reactive oxygen species (ROS) levels in young and senescent VECs before and after inhibiting PC-PLC activity. The results showed that suppressing PC-PLC inhibited apoptosis and the elevation of p53 expression induced by apoptosis in young cells, but not in senescent cells, and that inhibiting PC-PLC depressed intracellular ROS levels both in young and senescent cells. The data suggested that PC-PLC was involved in the association of apoptosis and senescence. Its function might be closely related to the level of p53 in VECs. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.