Singlet oxygen generation during the oxidation of L-tyrosine and L-dopa with mushroom tyrosinase
Singlet oxygen generation during the oxidation of L-tyrosine and L-dopa with mushroom tyrosinase
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DOI：
10.1016/j.bbrc.2016.02.056
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发表时间：
2016-03-18
影响因子：
3.1
通讯作者：
Baba, Toshihide
中科院分区：
文献类型：
--
作者：
Miyaji, Akimitsu;Kohno, Masahiro;Baba, Toshihide
本文研究了在磷酸盐缓冲液(pH 7.4)中，蘑菇酪氨酸酶氧化酪氨酸和L-多巴时产生单线态氧的现象。反应在单线态氧受体2，2，6，6-四甲基-4-哌啶酮(4-oxo-TEMP)存在下进行，并测定了自旋加合物4-oxo-2，2，6，6-四甲基-1-哌啶氧(4-oxo-TEMPO)的电子自旋共振谱(ESR)。在酪氨酸和L-多巴被酪氨酸酶氧化的过程中，4-氧-TEMPO引起的ESR信号增强，表明产生了单线态氧。结果表明，黑素细胞通过酪氨酸酶催化的黑色素合成产生O-1(2)。(C)2016 Elsevier Inc.保留所有权利。
The generation of singlet oxygen during the oxidation of tyrosine and L-dopa using mushroom tyrosinase in a phosphate buffer (pH 7.4), the model of melanin synthesis in melanocytes, was examined. The reaction was performed in the presence of 2,2,6,6-tetramethyl-4-piperidone (4-oxo-TEMP), an acceptor of singlet oxygen and the electron spin resonance (ESR) of the spin adduct, 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy (4-oxo-TEMPO), was measured. An increase in the ESR signal attributable to 4-oxo-TEMPO was observed during the oxidation of tyrosine and L-dopa with tyrosinase, indicating the generation of singlet oxygen. The results suggest that O-1(2) generation via tyrosinase-catalyzed melanin synthesis occurs in melanocyte. (C) 2016 Elsevier Inc. All rights reserved.