Heterocyst pattern formation controlled by a diffusible peptide
Heterocyst pattern formation controlled by a diffusible peptide
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DOI：
10.1126/science.282.5390.935
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发表时间：
1998-10-30
期刊：
影响因子：
56.9
通讯作者：
Golden, JW
中科院分区：
文献类型：
--
作者：
Yoon, HS;Golden, JW
许多丝状蓝藻作为多细胞生物生长，显示出由大约10个营养细胞分开的单个固氮异囊的发育模式。54碱基对基因patS的过表达抑制了Anabaena sp.菌株pcc7120的异囊分化。patS零突变体显示异囊频率增加和异常模式。patS-gfp报告基因的表达局限于发育中的原异囊。添加与PatS的后5个氨基酸相对应的合成肽抑制了杂种囊的发育。PatS似乎通过细胞间信号机制控制异囊模式的形成。
Many filamentous cyanobacteria grow as multicellular organisms that show a developmental pattern of single nitrogen-fixing heterocysts separated by approximately 10 vegetative cells. Overexpression of a 54-base-pair gene, patS, blocked heterocyst differentiation in Anabaena sp. strain PCC 7120. A patS null mutant showed an increased frequency of heterocysts and an abnormal pattern. Expression of a patS-gfp reporter was Localized in developing proheterocysts. The addition of a synthetic peptide corresponding to the Last five amino acids of PatS inhibited heterocyst development. PatS appears to control heterocyst pattern formation through intercellular signaling mechanisms.