Functional microdomains in bacterial membranes
Functional microdomains in bacterial membranes
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DOI：
10.1101/gad.1945010
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发表时间：
2010-09-10
影响因子：
10.5
通讯作者：
Kolter, Roberto
中科院分区：
文献类型：
--
作者：
Lopez, Daniel;Kolter, Roberto
真核细胞的膜具有称为脂筏的微结构域，其中含有多种信号传导和转运蛋白。在这里，我们证明细菌膜含有功能类似于真核细胞的微结构域。这些来自不同细菌的膜微结构域含有 Flotillin-1（一种仅在脂筏中发现的真核蛋白）的同源物，以及参与信号传导和运输的蛋白。通过萨拉哥酸（一种已知的角鲨烯合酶抑制剂）的作用抑制脂筏形成，从而损害生物膜形成和蛋白质分泌，但不损害细胞活力。微域中生理过程的协调可能是膜的一个比以前认识到的更广泛的特征。
The membranes of eukaryotic cells harbor microdomains known as lipid rafts that contain a variety of signaling and transport proteins. Here we show that bacterial membranes contain microdomains functionally similar to those of eukaryotic cells. These membrane microdomains from diverse bacteria harbor homologs of Flotillin-1, a eukaryotic protein found exclusively in lipid rafts, along with proteins involved in signaling and transport. Inhibition of lipid raft formation through the action of zaragozic acid-a known inhibitor of squalene synthases-impaired biofilm formation and protein secretion but not cell viability. The orchestration of physiological processes in microdomains may be a more widespread feature of membranes than previously appreciated.