Sperm storage and antioxidative enzyme expression in the honey bee, Apis mellifera
Sperm storage and antioxidative enzyme expression in the honey bee, Apis mellifera
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DOI：
10.1111/j.0962-1075.2004.00469.x
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发表时间：
2004-04-01
影响因子：
2.6
通讯作者：
Evans, JD
中科院分区：
文献类型：
--
作者：
Collins, AM;Williams, V;Evans, JD
蜜蜂的精子在交配的雌性蜜蜂的受精囊中仍能存活数年。在此期间，精子保持呼吸活动，使其面临许多生物过程中常见的活性氧物种的破坏作用。抗氧化酶可能有助于减轻这种损害。在这里，我们使用实时定量RT-PCR技术建立了三种抗氧化酶：过氧化氢酶、谷胱甘肽-S转移酶和超氧化物歧化酶在雄蜂和雌蜂繁殖组织中的基因表达谱。过氧化氢酶和谷胱甘肽转移酶在交配的蜂王的精子存储器官中的转录水平是未交配的蜂王的10到20倍，而SOD1水平在交配和未交配的蜂王中都很高。男性生殖和躯体组织显示，所有三种抗氧化剂编码转录本的水平都相对较高。所有这三种酶在成熟男性中都高于年轻男性，尽管这种影响似乎并不局限于生殖组织，因此不需要反映出在精子寿命中的作用。此外，在精子成熟和精液产生后很长一段时间内，男性组织中抗氧化物酶的转录仍然存在，而且明显增加。我们还在蜜蜂精液中发现了可测量的过氧化氢酶转录本。在蜜蜂的生殖组织和精液中都存在过氧化氢酶转录本，这表明该酶可能在抗氧化保护中发挥关键作用。
Honey bee (Apis mellifera) sperm remains viable in the spermatheca of mated female honey bees for several years. During this time, the sperm retains respiratory activity, placing it at risk of the damaging effects of reactive oxygen species common to many biological processes. Antioxidative enzymes might help reduce this damage. Here we use quantitative real-time RT-PCR to establish gene-expression profiles in male and female honey bee reproductive tissues for three antioxidative enzymes: catalase, glutathione-S-transferase (GST) and superoxide dismutase (SOD1, cytosolic). Catalase and GST showed ten- to twenty-fold transcript increases in the sperm storage organs of mated queens vs. unmated queens, whereas SOD1 levels are high in both mated and unmated queens. Male reproductive and somatic tissues showed relatively high levels of all three antioxidant-encoding transcripts. All three enzymes screened were higher in mature males vs. young males, although this effect did not appear to be confined to reproductive tissues and, hence, need not reflect a role in sperm longevity. Furthermore, antioxidative enzyme transcripts remained present, and apparently increased, in male tissues long after sperm had matured and seminal fluid was produced. We also found measurable levels of catalase transcripts in honey bee semen. The presence of catalase transcripts in both reproductive tissues and semen in bees suggests that this enzyme might play a key role in antioxidative protection.