RNA-template dependent de novo telomere addition
RNA-template dependent de novo telomere addition
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DOI：
10.1080/15476286.2015.1134414
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发表时间：
2016-01-01
期刊：
影响因子：
4.1
通讯作者：
Lipps, Hans J.
中科院分区：
文献类型：
--
作者：
Fuhrmann, Gloria;Joensson, Franziska;Lipps, Hans J.
端粒序列的从头添加可以发生在断裂的染色体上，并且必须得到很好的控制，这在程序化的DNA重组过程中是必不可少的。在纤毛虫原生动物中，在有性生殖后的大核分化过程中观察到一种极端形式的DNA重组，导致生殖细胞基因组的特定部分被消除。调节这些过程涉及小的非编码RNA，但另外DNA重排、切除和扩增需要来自亲本大核的RNA模板。我们发现，这些假定的RNA模板可以携带端粒重复序列。将携带修饰的端粒的RNA模板显微注射到发育中的大核中导致营养细胞中的修饰的端粒，这提供了强有力的证据，即端粒的从头添加取决于来自亲本大核的含有端粒的转录物。
De novo addition of telomeric sequences can occur at broken chromosomes and must be well controlled, which is essential during programmed DNA reorganization processes. In ciliated protozoa an extreme form of DNA-reorganization is observed during macronuclear differentiation after sexual reproduction leading to the elimination of specific parts of the germline genome. Regulating these processes involves small noncoding RNAs, but in addition DNA-reordering, excision and amplification require RNA templates deriving from the parental macronucleus. We show that these putative RNA templates can carry telomeric repeats. Microinjection of RNA templates carrying modified telomeres into the developing macronucleus leads to modified telomeres in vegetative cells, providing strong evidence, that de novo addition of telomeres depends on a telomere-containing transcript from the parental macronucleus.