Biofuels. Engineering alcohol tolerance in yeast.
Biofuels. Engineering alcohol tolerance in yeast.
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DOI：
10.1126/science.1257859
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发表时间：
2014-10-03
期刊：
影响因子：
--
通讯作者：
Stephanopoulos G
中科院分区：
文献类型：
--
作者：
Lam FH;Ghaderi A;Fink GR;Stephanopoulos G
Ethanol toxicity in yeast Saccharomyces cerevisiae limits titer and productivity in the industrial production of transportation bioethanol. We show that strengthening the opposing potassium and proton electrochemical membrane gradients is a mechanism that enhances general resistance to multiple alcohols. Elevation of extracellular potassium and pH physically bolster these gradients, increasing tolerance to higher alcohols and ethanol fermentation in commercial and laboratory strains (including a xylose-fermenting strain) under industrial-like conditions. Production per cell remains largely unchanged with improvements deriving from heightened population viability. Likewise, up-regulation of the potassium and proton pumps in the laboratory strain enhances performance to levels exceeding industrial strains. Although genetically complex, alcohol tolerance can thus be dominated by a single cellular process, one controlled by a major physicochemical component but amenable to biological augmentation.
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影响因子：
7
作者：
Swinnen S;Schaerlaekens K;Pais T;Claesen J;Hubmann G;Yang Y;Demeke M;Foulquié-Moreno MR;Goovaerts A;Souvereyns K;Clement L;Dumortier F;Thevelein JM
通讯作者：
Thevelein JM
DOI：
10.1093/dnares/dsr029
发表时间：
2011-12
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
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通讯作者：
Shimoi H
影响因子：
3.9
作者：
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通讯作者：
Bandyopadhyay, S
影响因子：
2.8
作者：
Katou, Taku;Namise, Masahiro;Shimoi, Hitoshi
通讯作者：
Shimoi, Hitoshi
影响因子：
2.6
作者：
van Voorst, F;Houghton-Larsen, J;Brandt, A
通讯作者：
Brandt, A