Overexpression of 20-oxidase confers a gibberellin-overproduction phenotype in Arabidopsis
Overexpression of 20-oxidase confers a gibberellin-overproduction phenotype in Arabidopsis
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DOI：
10.1104/pp.118.3.773
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发表时间：
1998-11-01
期刊：
影响因子：
7.4
通讯作者：
Brown, SM
中科院分区：
文献类型：
--
作者：
Huang, SS;Raman, AS;Brown, SM
在赤霉素（CA）生物合成途径中，20-氧化酶催化碳-20的氧化和消除以产生C（19）-GAs。所有生物活性GA都是C（19）-GA。我们在转基因拟南芥植物中过表达了编码从拟南芥幼苗中分离的20-氧化酶的cDNA。这些转基因植物表现出可能归因于CA过量产生的表型。该表型包括下胚轴更长、叶片更浅绿、茎伸长增加、开花更早和种子休眠减少。然而，转基因植物的生育力不受影响。在检测的转基因系的幼苗中检测到内源GA（1）、GA（9）和GA（20）水平增加。GA（4）被认为是拟南芥中主要的活性GA，在该品系中不以增加的水平存在。这些结果表明，这种20-氧化酶的过量表达增加了转基因幼苗中的一些内源GAs的水平，这导致了GA过量生产表型。
In the gibberellin (CA) biosynthesis pathway, 20-oxidase catalyzes the oxidation and elimination of carbon-20 to give rise to C(19)-GAs. All bioactive GAs are C(19)-GAs. We have overexpressed a cDNA encoding 20-oxidase isolated from Arabidopsis seedlings in transgenic Arabidopsis plants. These transgenic plants display a phenotype that may be attributed to the overproduction of CA. The phenotype includes a longer hypocotyl, lighter-green leaves, increased stem elongation, earlier flowering, and decreased seed dormancy. However, the fertility of the transgenic plants is not affected. Increased levels of endogenous GA(1), GA(9), and GA(20), were detected in seedlings of the transgenic line examined. GA(4), which is thought to be the predominantly active GA in Arabidopsis, was not present at increased levels in this line. These results suggest that the overexpression of this 20-oxidase increases the levels of some endogenous GAs in transgenic seedlings, which causes the GA-overproduction phenotype.