Calorie restriction and ketogenic diet diminish neuronal excitability in rat dentate gyrus in vivo
Calorie restriction and ketogenic diet diminish neuronal excitability in rat dentate gyrus in vivo
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DOI：
10.1046/j.1528-1157.2003.55502.x
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发表时间：
2003-06-01
期刊：
影响因子：
5.6
通讯作者：
Rho, JM
中科院分区：
文献类型：
--
作者：
Bough, KJ;Schwartzkroin, PA;Rho, JM
目的：生酮饮食（KD）是治疗难治性癫痫的有效方法。然而，鲜为人知的是，其潜在的mechanism.Methods：在这项研究中，在体内细胞外场角束刺激的反应记录在Sprague-Dawley大鼠喂三种饮食之一：生酮热量限制（KCR），正常热量限制（NCR），或正常随意（NAL）的齿状回。输入/输出曲线和成对脉冲关系被用来评估网络的兴奋性。A最大齿状激活（MDA）协议被用来测量电图癫痫发作阈值和duration.Results：动物喂养的热量限制（CR）饮食表现出更大的成对脉冲抑制，NIDA阈值升高，和没有蔓延抑郁样事件相比，随意喂养的控制。在MDA模型的癫痫，电图癫痫发作持续时间的增加率在反复刺激后显着减少KCR喂养的动物相比，NCR和NAL喂养controls.Conclusions：这些数据表明，CR本身，可以抗惊厥，和治疗与KCR饮食可能是抗惊厥和抗癫痫。
Purpose: The ketogenic diet (KD) is an effective treatment for intractable epilepsy. However, little is known about its underlying mechanisms.Methods: In this study, in vivo extracellular field responses to angular bundle stimulation were recorded in the dentate gyrus of Sprague-Dawley rats fed one of three diets: ketogenic calorie-restricted (KCR), normal calorie-restricted (NCR), or normal ad libitum (NAL). Input/output curves and paired-pulse relations were used to assess network excitability. A maximal dentate activation (MDA) protocol was used to measure electrographic seizure threshold and duration.Results: Animals fed calorie-restricted (CR) diets exhibited greater paired-pulse inhibition, an elevated NIDA threshold, and an absence of spreading depression-like events compared with ad libitum-fed controls. In the MDA model of epileptogenesis, the rate of increase in electrographic seizure duration after repeated stimuli was markedly reduced in KCR-fed animals compared with NCR- and NAL-fed controls.Conclusions: These data suggest that CR, by itself, can be anticonvulsant, and treatment with a KCR diet may be both anticonvulsant and antiepileptogenic.