Lycopene oxidation product enhances gap junctional communication
Lycopene oxidation product enhances gap junctional communication
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DOI：
10.1016/s0278-6915(03)00148-0
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发表时间：
2003-10-01
影响因子：
4.3
通讯作者：
Stahl, W
中科院分区：
文献类型：
--
作者：
Aust, O;Ale-Agha, N;Stahl, W
类胡萝卜素及其代谢物和氧化产物刺激细胞之间的间隙连接通讯（GJC），这被认为是这些化合物预防癌症活性相关的保护机制之一。在流行病学上，通过食用番茄或番茄制品而增加番茄红素的摄入量与前列腺癌风险降低有关。在这里，我们报道了番茄红素氧化产物对大鼠肝上皮WB-F344细胞GJC的刺激作用。用过氧化氢/四氧化锇对番茄红素进行体外完全氧化，得到活性化合物。结构分析采用高效液相色谱法、气相色谱联用质谱法、紫外/可见光和红外分光光度法。生物活性氧化产物鉴定为2,7,11 -三甲基十四己烯- 1,14 -dial。目前的数据表明，番茄红素降解产物在细胞信号传导中的潜在作用，通过间隙连接增强细胞间的通信。(C) 2003 Elsevier Ltd.版权所有。
Carotenoids as well as their metabolites and oxidation products stimulate gap junctional communication (GJC) between cells, which is thought to be one of the protective mechanisms related to cancer-preventive activities of these compounds. Increased intake of lycopene by consumption of tomatoes or tomato products has been epidemiologically associated with a diminished risk of prostate cancer. Here, we report a stimulatory effect of a lycopene oxidation product on GJC in rat liver epithelial WB-F344 cells. The active compound was obtained by complete in vitro oxidation of lycopene with hydrogen peroxide/osmium tetroxide. For structural analysis high performance liquid chromatography, gas chromatography coupled with mass spectrometry, ultraviolet/visible-, and infrared spectrophotometry were applied. The biologically active oxidation product was identified as 2,7,1 1-trimethyltetradecahexaene-1, 14-dial. The present data indicate a potential role of lycopene degradation products in cell signaling enhancing cell-to-cell communication via gap junctions. (C) 2003 Elsevier Ltd. All rights reserved.