SPECIFICITY OF MONOCLONAL-ANTIBODIES AGAINST GROUP-B STREPTOCOCCUS TYPE-II AND INHIBITION OF THEIR BINDING BY HUMAN SECRETIONS
SPECIFICITY OF MONOCLONAL-ANTIBODIES AGAINST GROUP-B STREPTOCOCCUS TYPE-II AND INHIBITION OF THEIR BINDING BY HUMAN SECRETIONS
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DOI：
10.1203/00006450-198807000-00017
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发表时间：
1988-07-01
影响因子：
3.6
通讯作者：
PRITCHARD, DG
中科院分区：
文献类型：
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作者：
GRAY, BM;EGAN, ML;PRITCHARD, DG
针对II型B链球菌的型特异性多糖抗原的小鼠单克隆抗体可根据其抗原结合特性分为两大类。一组抗体结合完整的和去唾液酸化的II型抗原，并且该结合可以被β-吡喃半乳糖苷第二组抗体仅与完整的II型多糖反应，并且不被β-葡聚糖抑制。吡喃半乳糖苷第一组抗体的结合也可以被与II型多糖具有结构相似性的正常人分泌物的碳水化合物组分抑制。一种特异性抗体的结合排除了另一种特异性抗体的结合，这显然是通过竞争包含两种决定簇的结合区域内的结合位点实现的。
Mouse monoclonal antibodies against the type-specific polysaccharide antigen of type II group B Streptococcus may be divided into two general groups based on their antigen-binding properties. One group of antibodies binds to both intact and desialylated type II antigen and the binding can be inhibited by .beta.-galactopyranosides. The second group of antibodies react only with intact type II polysaccharide and are not inhibited by .beta.-galactopyranosides. The binding of antibodies of the first group can also be inhibited by carbohydrate components of normal human secretions that share structural similarities with the type II polysaccharide. The binding of antibodies on one specificity precluded the binding of antibodies of the other specificity, apparently by competing for binding sites within a combining area that includes both determinants.