Activation of GABAergic Neurons in the Rostromedial Tegmental Nucleus and Other Brainstem Regions Promotes Sedation and Facilitates Sevoflurane Anesthesia in Mice.
Activation of GABAergic Neurons in the Rostromedial Tegmental Nucleus and Other Brainstem Regions Promotes Sedation and Facilitates Sevoflurane Anesthesia in Mice.
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DOI：
10.1213/ane.0000000000005387
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发表时间：
2021-04-01
影响因子：
5.7
通讯作者：
Solt K
中科院分区：
文献类型：
--
作者：
Vlasov K;Pei J;Nehs CJ;Guidera JA;Zhang ER;Kenny JD;Houle TT;Brenner GJ;Taylor NE;Solt K
许多全身麻醉药可增强 GABAA 受体，但其神经解剖学作用位点尚不清楚。嘴内侧被盖核 (RMTg) 中的 GABA 能神经元向多个促进唤醒的核团发送抑制性投射，但这些神经元在调节意识中的作用尚不清楚。在这项研究中，设计受体针对 Vgat-ires-Cre 小鼠的 RMTg GABA 能神经元。 DREADDs 在 RMTg 和其他脑干区域中表达。这些神经元的激活减少了运动和探索行为，损害了运动协调性，诱发了类似于非快速眼动睡眠的脑电图振荡，但没有失去正位，并减少了七氟烷引起的意识丧失的剂量需求。这些结果表明 RMTg 和其他脑干区域中的 GABA 能神经元可促进镇静并促进七氟醚引起的意识丧失。
Many general anesthetics potentiate GABAA receptors but their neuroanatomic sites of action are less clear. GABAergic neurons in the rostromedial tegmental nucleus (RMTg) send inhibitory projections to multiple arousal-promoting nuclei, but the role of these neurons in modulating consciousness is unknown. In this study, designer receptors were targeted to RMTg GABAergic neurons of Vgat-ires-Cre mice. DREADDs expression was found in the RMTg and other brainstem regions. Activation of these neurons decreased movement and exploratory behavior, impaired motor coordination, induced EEG oscillations resembling NREM sleep without loss of righting, and reduced the dose requirement for sevoflurane-induced unconsciousness. These results suggest that GABAergic neurons in the RMTg and other brainstem regions promote sedation and facilitate sevoflurane-induced unconsciousness.