Dietary β-Sitosterol Improves Growth Performance, Meat Quality, Antioxidant Status, and Mitochondrial Biogenesis of Breast Muscle in Broilers
Dietary β-Sitosterol Improves Growth Performance, Meat Quality, Antioxidant Status, and Mitochondrial Biogenesis of Breast Muscle in Broilers
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DOI：
10.3390/ani9030071
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发表时间：
2019-02-26
期刊：
影响因子：
3
通讯作者：
Zhou, Yanmin
中科院分区：
文献类型：
--
作者：
Cheng, Yefei;Chen, Yueping;Zhou, Yanmin
本研究评估了 β-谷甾醇对肉鸡生长性能、肉质、氧化状态和胸肌线粒体生物合成的影响。一天大的雏鸡被分配到五个处理组，每个处理组六个重复。肉鸡饲喂基础日粮，添加 0（对照）、40、60、80 或 100 mg/kg β-谷甾醇，持续 42 天。 β-谷甾醇线性和二次降低胸肌的料增比、亮度（24h）和烹饪损失（24h），而胸肌的2,2-二苯基-1-三硝基苯肼清除活性则呈现相反的趋势。 β-谷甾醇线性降低胸肌中的滴水损失（24小时）和丙二醛含量，而线性增加pH（24小时）、超氧化物歧化酶活性以及过氧化物酶体增殖物激活受体γ共激活剂1α（PCG-1α）和线粒体转录因子A（TFAM）的mRNA丰度。与对照相比，β-谷甾醇水平高于40 mg/kg会降低料重比、肌肉亮度（24小时）、蒸煮损失（24小时）和丙二醛水平，而增加肌肉2,2-二苯基-1-三硝基苯肼清除活性以及PCG-1α和TFAM的mRNA丰度（60 mg/kg除外）。 80毫克/千克β-谷甾醇增加肌肉pH值（24小时）和超氧化物歧化酶活性，但减少其滴水损失（24小时）。因此，β-谷甾醇可以改善肉鸡胸肌的生长性能和肉品质、氧化状态和线粒体生物合成。此外，建议肉鸡日粮中添加 80 mg/kg β-谷甾醇。
The present study evaluated effects of beta-sitosterol on growth performance, meat quality, oxidative status, and mitochondrial biogenesis of breast muscle in broilers. One-day-old chicks were allocated to five treatments of six replicates. Broilers were fed a basal diet supplemented either with 0 (control), 40, 60, 80, or 100 mg/kg beta-sitosterol for 42 days. beta-sitosterol linearly and quadratically reduced feed/gain ratio, lightness(24h) and cooking loss(24h) in breast muscle, whereas 2, 2-diphenyl-1-picrylhydrazyl scavenging activity of breast muscle followed an opposite trend. beta-sitosterol linearly decreased drip loss(24h) and malondialdehyde content, whereas linearly increased pH(24h), superoxide dismutase activity, and mRNA abundances of peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PCG-1 alpha) and mitochondrial transcription factor A (TFAM) in breast muscle. Compared with control, levels of beta-sitosterol higher than 40 mg/kg reduced feed/gain ratio, muscular lightness(24h), cooking loss(24h), and malondialdehyde level, whereas increased muscular 2, 2-diphenyl-1-picrylhydrazyl scavenging activity, and mRNA abundances (except 60 mg/kg) of PCG-1 alpha and TFAM. Eighty milligram/kilogram beta-sitosterol increased muscular pH(24h) and superoxide dismutase activity, but decreased its drip loss(24h). Therefore, beta-sitosterol could improve growth performance and meat quality, oxidative status, and mitochondrial biogenesis of breast muscle in broilers. Furthermore, supplementation level of 80 mg/kg beta-sitosterol is recommended for broiler diets.