A new light-scattering sensor for screening G-quadruplex stabilizers based on DNA-folding-mediated assembly of gold nanoparticles
A new light-scattering sensor for screening G-quadruplex stabilizers based on DNA-folding-mediated assembly of gold nanoparticles
复制标题
一种基于 DNA 折叠介导的金纳米颗粒组装筛选 G 四链体稳定剂的新型光散射传感器
DOI：
10.1039/c3tb20291g
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发表时间：
2013
影响因子：
7
通讯作者：
Li Dan
中科院分区：
文献类型：
--
作者：
Feng Da-Qian;Liu Guoliang;Zheng Wenjie;Chen Tianfeng;Li Dan
在这篇论文中，我们利用光散射技术构建了一种筛选g -四联体稳定剂的生物传感器，该传感器基于富鸟嘌呤（G-rich） ssDNA功能化金纳米颗粒的组装。我们合成了一系列金属-三联吡啶配合物，并利用生物传感器研究了它们对四联体dna的亲和力。利用光散射峰强度(I−I0)/I0的比值，可以直观地呈现出g -四重体DNA稳定能力的序列为：复合物1 >复合物3 > MTX >复合物2 >复合物5 >复合物6 >复合物7 >复合物4 >甲基绿> TO >复合物8 >顺铂。由于我们的方法可以定量分析稳定剂的亲和力，因此对配合物1、配合物3、MTX、配合物2、配合物5、配合物6、配合物7、配合物4、甲基绿、TO和配合物8的EC50值分别为4.8、5.3、6.1、6.6、6.7、18.2、20.2、21.5、32.2、41.5和48.1 μM。结果经g -四联荧光插层置换（G4-FID）分析证实。该方法是一种简单、方便、直观、高灵敏度的g -四联稳定剂筛选方法。我们开发的生物传感器为筛选抗癌药物提供了一个很有前途的工具。
In this contribution, we constructed a biosensor for screening G-quadruplex stabilizers based on the assembly of gold nanoparticles functionalized with guanine-rich (G-rich) ssDNA using a light-scattering technique. We synthesized a series of metal–terpyridine complexes and investigated their affinity for quadruplex-DNA using the biosensor. Using the ratio of intensity of the light-scattering peak (I − I0)/I0, it can intuitively present the sequence of ability of stabilizing G-quadruplex DNA as follows: complex 1 > complex 3 > MTX > complex 2 > complex 5 > complex 6 > complex 7 > complex 4 > methyl green > TO > complex 8 > cisplatin. As our method allows the quantitative analysis of stabilizer affinity, EC50 values obtained are 4.8, 5.3, 6.1, 6.6, 6.7, 18.2, 20.2, 21.5, 32.2, 41.5 and 48.1 μM for complex 1, complex 3, MTX, complex 2, complex 5, complex 6, complex 7, complex 4, methyl green, TO and complex 8, respectively. The results have been verified by G-quadruplex fluorescent intercalator displacement (G4-FID) analysis. The proposed approach is a simple, convenient, intuitive and highly sensitive assay for screening G-quadruplex stabilizers. Our developed biosensor provides a promising tool for screening anticancer drugs.