ACUTE TOXICITY MEASUREMENTS ON AQUATIC POLLUTANTS USING MICROCALORIMETRY ON TISSUE-CULTURED CELLS
ACUTE TOXICITY MEASUREMENTS ON AQUATIC POLLUTANTS USING MICROCALORIMETRY ON TISSUE-CULTURED CELLS
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DOI：
10.1021/es00018a012
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发表时间：
1991-06-01
影响因子：
11.4
通讯作者：
BARISAS, BG
中科院分区：
文献类型：
--
作者：
MCGUINNESS, SM;BARISAS, BG
一种新的急性细胞毒性微热量生物测定方法是基于组织培养的人细胞的代谢热演变。该分析是定量的、廉价的和通用的；此外，毒理学信息可以从其他感兴趣的物种的细胞中获得。对毒物的生物反应是抑制细胞中的产热，毒性表示为在这种抑制中有效的浓度50% （EC50）。因此，该系统可以测量在各种细胞代谢途径中具有靶点的多种毒物的急性毒性。HeLa细胞的平均发热量为40 pW/细胞，再现性为5 pW/细胞。细胞样品的范围从8 × 10(5)到2 × 10(6)个细胞，在没有毒物的情况下产生30-80 μ w的信号。这些信号很容易在LKB 10700-1型流量微热量计中测量。结果与Beckman Microtox生物测定法、大肠杆菌电极法和虹鳟鱼96 h LC50值进行了比较。
A new microcalorimetric bioassay for acute cellular toxicity is based on metabolic heat evolution from tissue-cultured human cells. The assay is quantitative, inexpensive, and versatile; moreover, toxicological information can be obtained with cells from other species of interest. The biological response to toxicants is inhibition of heat production in cells and toxicity is expressed as the concentration 50% effective in this inhibition (EC50). The system can therefore measure acute toxicity for a broad range of toxicants having target sites in various cellular metabolic pathways. Average heat production in HeLa cells is 40 pW/cell and the reproducibility is 5 pW/cell. Cell samples range from 8 x 10(5) to 2 x 10(6) cells and yield signals of 30-80-mu-W in the absence of toxicants. These signals are readily measurable in an LKB Model 10700-1 flow microcalorimeter. Results are compared with those obtained with the Beckman Microtox bioassay and the Escherichia coli electrode and with rainbow trout 96-h LC50 values.