"Superchiral" Spectroscopy: Detection of Protein Higher Order Hierarchical Structure with Chiral Plasmonic Nanostructures
"Superchiral" Spectroscopy: Detection of Protein Higher Order Hierarchical Structure with Chiral Plasmonic Nanostructures
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DOI：
10.1021/jacs.5b04806
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发表时间：
2015-07-08
影响因子：
15
通讯作者：
Kadodwala, Malcolm
中科院分区：
文献类型：
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作者：
Tullius, Ryan;Karimullah, Affar S.;Kadodwala, Malcolm
光谱学方法通常不能提供生物大分子和组装体的高阶层次结构（三级/四级）的信息。这就需要使用耗时且材料密集的技术，如蛋白质晶体学、核磁共振和电子显微镜。在这里，我们展示了一种光谱现象，超手性极化，它可以快速表征配体诱导的蛋白质高阶（三级/四级）结构在picogram水平上的变化，这是传统CD光谱无法检测到的。这是通过利用超手性倏逝场对中尺度手性结构的增强敏感性来实现的。
Optical spectroscopic methods do not routinely provide information on higher order hierarchical structure (tertiary/quaternary) of biological macromolecules and assemblies. This necessitates the use of time-consuming and material intensive techniques, such as protein crystallography, NMR, and electron microscopy. Here we demonstrate a spectroscopic phenomenon, superchiral polarimetry, which can rapidly characterize ligand-induced changes in protein higher order (tertiary/quaternary) structure at the picogram level, which is undetectable using conventional CD spectroscopy. This is achieved by utilizing the enhanced sensitivity of superchiral evanescent fields to mesoscale chiral structure.