Precise deletion of tagD and controlled depletion of its product, glycerol 3-phosphate cytidylyltransferase, leads to irregular morphology and lysis of Bacillus subtilis grown at physiological temperature
Precise deletion of tagD and controlled depletion of its product, glycerol 3-phosphate cytidylyltransferase, leads to irregular morphology and lysis of Bacillus subtilis grown at physiological temperature
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DOI：
10.1128/jb.183.22.6688-6693.2001
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发表时间：
2001-11-01
影响因子：
3.2
通讯作者：
Brown, ED
中科院分区：
文献类型：
--
作者：
Bhavsar, AP;Beveridge, TJ;Brown, ED
使用先前报道的用于枯草芽孢杆菌的条件表达系统，我们报道了B中磷壁酸生物合成（标签）基因tagD的首次精确缺失。枯草杆菌。该磷壁酸突变体在生理温度和确定的遗传背景下表现出致死表型。该tagD突变体在tagD的互补拷贝表达后进行完全表型拯救。耗尽的tagD基因产物（甘油3-磷酸胞苷酰转移酶）通过调制的tagD从amyE位点的表达揭示了结构缺陷集中在形状，分隔和分裂。在这些事件之后壁增厚并最终溶解。
Using a previously reported conditional expression system for use in Bacillus subtilis we report the first precise deletion of a teichoic acid biosynthesis (tag) gene, tagD, in B. subtilis. This teichoic acid mutant showed a lethal phenotype when characterized at a physiological temperature and in a defined genetic background. This tagD mutant was subject to full phenotypic rescue upon expression of the complementing copy of tagD. Depletion of the tagD gene product (glycerol 3-phosphate cytidylyltransferase) via modulated expression of tagD from the amyE locus revealed structural defects centered on shape, septation, and division. Thickening of the wall and ultimately lysis followed these events.