PERSISTENCE LENGTH OF RNA
PERSISTENCE LENGTH OF RNA
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DOI：
10.1021/bi00013a026
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发表时间：
1995-04-04
期刊：
影响因子：
2.9
通讯作者：
HAGERMAN, P
中科院分区：
文献类型：
--
作者：
KEBBEKUS, P;DRAPER, DE;HAGERMAN, P
一组10个双链RNA的长度范围从92至317碱基对已被合成，并通过瞬态电双折射测量其旋转扩散时间。双折射衰减数据的流体动力学分析产生720 +/-70埃的螺旋持续长度，基于在Mg 2+离子存在下具有26埃的有效流体动力学直径和2.7埃的每碱基对上升的螺旋。因此，双链体RNA比DNA稍硬，DNA的持久长度为450-500埃。测量结果还表明，（i）目前的旋转衰减时间的流体动力学理论适用于长于100个碱基对的RNA分子，（ii）弱Mg 2 +-RNA缔合使每个碱基对的上升略微降低，不超过0.1埃。
A set of 10 double-stranded RNAs ranging in length from 92 to 317 base pairs has been synthesized, and their rotational diffusion times were measured by transient electric birefringence. A hydrodynamic analysis oi the birefringence decay data yields a helix persistence length of 720 +/- 70 Angstrom, based on a helix with an effective hydrodynamic diameter of 26 Angstrom and a rise per base pair of 2.7 Angstrom in the presence of Mg2+ ions. Thus, duplex RNA is somewhat stiffer than DNA, for which the persistence length is 450-500 Angstrom. The measurements also suggest that (i) current hydrodynamic theory for the rotational decay times is applicable to RNA molecules that are longer than 100 base pairs and (ii) weak Mg2+-RNA association decreases the rise per base pair slightly, by no more than 0.1 Angstrom.