Proteomic analysis of the similarities and differences of soil drought and polyethylene glycol stress responses in wheat (Triticum aestivum L.)
Proteomic analysis of the similarities and differences of soil drought and polyethylene glycol stress responses in wheat (Triticum aestivum L.)
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DOI：
10.1007/s11103-019-00866-2
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发表时间：
2019-07-01
影响因子：
5.1
通讯作者：
Xi, Yajun
中科院分区：
文献类型：
--
作者：
Cui, Guibin;Zhao, Yanfeng;Xi, Yajun
结果表明，土壤干旱和聚乙二醇都能促进苹果酸、谷胱甘肽和抗坏血酸的代谢，以及脯氨酸的生物合成，而土壤干旱诱导这些代谢途径的程度比聚乙二醇更大。聚乙二醇被广泛用于模拟渗透胁迫，但对小麦对聚乙二醇盐胁迫和土壤干旱的不同响应知之甚少。本研究利用等压相对定量标签(ITRAQ)蛋白质组学技术，研究了小麦幼苗对土壤干旱和聚乙二醇乙二醇酯(PEG)的蛋白质组学和生理响应。结果表明，光合作用速率、气孔导度、胞间CO2浓度、蒸腾速率、PSⅡ最大潜在效率、叶片含水量、相对电解质渗漏率、丙二醛含量和游离脯氨酸含量对土壤干旱和聚乙二醇的响应相似。约15.8%的差异蛋白质同时受到土壤干旱和聚乙二醇乙二醇酯的诱导。此外，土壤干旱和聚乙二醇均通过改变甘油醛-3-磷酸脱氢酶、核酮-二磷酸羧基酶和磷酸甘油酸激酶的积累来抑制碳代谢和某些氨基酸的生物合成，但都通过增加苹果酸脱氢酶、单脱氢抗坏血酸还原酶、吡咯烷-5-羧酸脱氢酶、吡咯烷-5-羧酸合成酶、抗坏血酸过氧化物酶、谷胱甘肽过氧化物酶和谷胱甘肽S转移酶等关键酶的积累来促进苹果酸、脯氨酸、谷胱甘肽和抗坏血酸的代谢。值得注意的是，后五种酶被发现对土壤干旱更敏感。此外，聚乙二醇胁迫下多胺氧化酶和亚精胺合成酶基因表达和蛋白积累的增加是诱导多胺生物合成的特异性原因，而果糖-二磷酸缩醛酶和精氨酸酶则受土壤干旱诱导。因此，目前的研究结果表明，聚乙二醇法是模拟干旱胁迫的一种有效方法，但在土壤干旱条件下，与苹果酸、谷胱甘肽、抗坏血酸、脯氨酸和多胺代谢相关的关键蛋白质还有待确定。
Key messageOur results reveal both soil drought and PEG can enhance malate, glutathione and ascorbate metabolism, and proline biosynthesis, whereas soil drought induced these metabolic pathways to a greater degree than PEG.AbstractPolyethylene glycol (PEG) is widely used to simulate osmotic stress, but little is known about the different responses of wheat to PEG stress and soil drought. In this study, isobaric tags for relative quantification (iTRAQ)-based proteomic techniques were used to determine both the proteomic and physiological responses of wheat seedlings to soil drought and PEG. The results showed that photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, maximum potential efficiency of PS II, leaf water content, relative electrolyte leakage, MDA content, and free proline content exhibited similar responses to soil drought and PEG. Approximately 15.8% of differential proteins were induced both by soil drought and PEG. Moreover, both soil drought and PEG inhibited carbon metabolism and the biosynthesis of some amino acids by altering the accumulation of glyceraldehyde-3-phosphate dehydrogenase, ribulose-bisphosphate carboxylase, and phosphoglycerate kinase, but they both enhanced the metabolism of malate, proline, glutathione, and ascorbate by increasing the accumulation of key enzymes including malate dehydrogenase, monodehydroascorbate reductase, pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylate synthetase, ascorbate peroxidase, glutathione peroxidase, and glutathione S-transferase. Notably, the latter five of these enzymes were found to be more sensitive to soil drought. In addition, polyamine biosynthesis was specifically induced by increased gene expression and protein accumulation of polyamine oxidase and spermidine synthase under PEG stress, whereas fructose-bisphosphate aldolase and arginase were induced by soil drought. Therefore, present results suggest that PEG is an effective method to simulate drought stress, but the key proteins related to the metabolism of malate, glutathione, ascorbate, proline, and polyamine need to be confirmed under soil drought.