An amino acid mixture improves glucose tolerance and insulin signaling in Sprague-Dawley rats
An amino acid mixture improves glucose tolerance and insulin signaling in Sprague-Dawley rats
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DOI：
10.1152/ajpendo.00643.2010
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发表时间：
2011-04-01
影响因子：
5.1
通讯作者：
Ivy, John L.
中科院分区：
文献类型：
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作者：
Bernard, Jeffrey R.;Liao, Yi-Hung;Ivy, John L.
Bernard JR，Liao YH，Hara D，Ding Z，Chen CY，纳尔逊尔逊JL，Ivy JL.氨基酸混合物改善Sprague-Dawley大鼠的葡萄糖耐量和胰岛素信号传导。Am J Physiol Endocrinol Metab 300：E752-E760，2011。首次出版于2011年2月8日; doi：10.1152/ajpendo. 00643.2010.-本研究的目的是评估氨基酸补充剂对口服葡萄糖挑战的葡萄糖反应的影响（实验1），并评估血糖反应的差异是否与骨骼肌葡萄糖摄入增加有关（实验2）。实验1大鼠用葡萄糖（CHO）、葡萄糖加氨基酸混合物（CHO-AA-1）、葡萄糖加亮氨酸浓度增加的氨基酸混合物（CHO-AA-2）或水（PLA）管饲。与CHO相比，CHO-AA-1和CHO-AA-2的血糖反应降低，这些治疗之间的胰岛素无差异。实验2大鼠用CHO或CHO-AA-1管饲。灌胃后15分钟，通过尾静脉输注含2-[H-3]脱氧葡萄糖和[U-C-14]甘露醇的推注。CHO-AA-1中的血糖显著低于CHO，而胰岛素反应相似。在快缩红肌（8.36 +/- 1.3 vs. 5.27 +/- 0.7 μ mol.g（-1）.h（-1））和白色肌（1.85 +/- 0.3 vs. 1.11 +/- 0.2 μ mol.g（-1）.h（-1））中，CHO-AA-1的肌肉葡萄糖摄取高于CHO。治疗组之间Akt/PKB磷酸化没有差异;然而，氨基酸治疗导致两种肌纤维类型中AS 160磷酸化增加。与CHO相比，CHO-AA-1的快缩红肌中糖原合酶磷酸化减少，而mTOR磷酸化增加。这些差异在快速收缩白色肌中没有发现。这些发现表明，氨基酸补充剂可以通过增加骨骼肌葡萄糖摄取和通过增强的细胞内信号转导的细胞内处置来改善葡萄糖耐量。
Bernard JR, Liao YH, Hara D, Ding Z, Chen CY, Nelson JL, Ivy JL. An amino acid mixture improves glucose tolerance and insulin signaling in Sprague-Dawley rats. Am J Physiol Endocrinol Metab 300: E752-E760, 2011. First published February 8, 2011; doi: 10.1152/ajpendo. 00643.2010.-The aims of this investigation were to evaluate the effect of an amino acid supplement on the glucose response to an oral glucose challenge (experiment 1) and to evaluate whether differences in blood glucose response were associated with increased skeletal muscle glucose uptake (experimental 2). Experiment 1 rats were gavaged with either glucose (CHO), glucose plus an amino acid mixture (CHO-AA-1), glucose plus an amino acid mixture with increased leucine concentration (CHO-AA-2), or water (PLA). CHO-AA-1 and CHO-AA-2 had reduced blood glucose responses compared with CHO, with no difference in insulin among these treatments. Experiment 2 rats were gavaged with either CHO or CHO-AA-1. Fifteen minutes after gavage, a bolus containing 2-[H-3] deoxyglucose and [U-C-14] mannitol was infused via a tail vein. Blood glucose was significantly lower in CHO-AA-1 than in CHO, whereas insulin responses were similar. Muscle glucose uptake was higher in CHO-AA-1 compared with CHO in both fast-twitch red (8.36 +/- 1.3 vs. 5.27 +/- 0.7 mu mol.g(-1).h(-1)) and white muscle (1.85 +/- 0.3 vs. 1.11 +/- 0.2 mu mol.g(-1).h(-1)). There was no difference in Akt/PKB phosphorylation between treatment groups; however, the amino acid treatment resulted in increased AS160 phosphorylation in both muscle fiber types. Glycogen synthase phosphorylation was reduced in fast-twitch red muscle of CHO-AA-1 compared with CHO, whereas mTOR phosphorylation was increased. These differences were not noted in fast-twitch white muscle. These findings suggest that amino acid supplementation can improve glucose tolerance by increasing skeletal muscle glucose uptake and intracellular disposal through enhanced intracellular signaling.