ULTRASTRUCTURAL DISCRIMINATION OF LIPID DROPLETS AND VESICLES IN ATHEROSCLEROSIS - VALUE OF OSMIUM THIOCARBOHYDRAZIDE OSMIUM AND TANNIC-ACID PARAPHENYLENEDIAMINE TECHNIQUES
ULTRASTRUCTURAL DISCRIMINATION OF LIPID DROPLETS AND VESICLES IN ATHEROSCLEROSIS - VALUE OF OSMIUM THIOCARBOHYDRAZIDE OSMIUM AND TANNIC-ACID PARAPHENYLENEDIAMINE TECHNIQUES
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DOI：
10.1177/36.10.2458408
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发表时间：
1988-10-01
影响因子：
3.2
通讯作者：
KLEMP, KF
中科院分区：
文献类型：
--
作者：
GUYTON, JR;KLEMP, KF
动脉粥样硬化心房组织的电子显微镜通常无法区分脂质囊泡和液滴，特别是当它们存在于细胞外空间时。这种区别很重要，因为囊泡或膜状脂质由磷脂和未酯化的胆固醇组成，而液滴形式的中性脂质意味着动脉粥样硬化中胆固醇酯的存在。一种对锇组织进行连续单宁酸和对苯二胺处理的新程序 [TA-PDA] 可以对脂质囊泡和液滴进行可靠的超微结构区分。许多囊泡的多层特征被揭示。人们发现细胞外液滴具有许多与膜泡相关的表面凹坑。在使用替代组织处理技术（整体应用锇-硫代碳酰肼-锇 (OTO) 序列）后，液滴的点蚀尤其明显。这两种互补技术将被证明对于动脉粥样硬化和其他富含脂质的组织的电子显微镜研究有用。
Electron microscopy of atherosclerotic atrial tissue commonly fails to distinguish lipid vesicles from droplets, especially when these are found in the extracellular space. The distinction is important, because vesicular or membranous lipid is composed of phospholipid and unesterified cholesterol, whereas neutral lipid in droplet form implies the pressence of cholesteryl ester in atherosclerosis. A new procedure with sequential tannic acid and p-phenylenediamine treatments of osmicated tissue [TA-PDA] allows reliable ultrastructural discrimination of lipid vescles and droplets. The multilamellar character of many vesicles is revealed. Extracellular droplets are found to posses many surface pits associated with membranous blebs. Pitting of droplets is especially evident after the use of an alternative tissue processing technique, the osmium-thiocarbohydrazide-osmium (OTO) sequence applied en bloc. The two complementary techniques will prove useful for electron microscopic studies of atherosclerotic and other lipid-rich tissues.