Aspirin eugenol ester regulates cecal contents metabolomic profile and microbiota in an animal model of hyperlipidemia
Aspirin eugenol ester regulates cecal contents metabolomic profile and microbiota in an animal model of hyperlipidemia
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DOI：
10.1186/s12917-018-1711-x
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发表时间：
2018-12-18
影响因子：
2.6
通讯作者：
Li, Jian-Yong
中科院分区：
文献类型：
--
作者：
Ma, Ning;Liu, Xi-Wang;Li, Jian-Yong
背景高脂血症的发病率越来越高，已成为伴侣动物临床常见的代谢性疾病之一。阿司匹林丁香酚酯（AEE）是一种具有抗高脂血症活性的新型化合物。然而，其机制仍不完全清楚。本研究旨在探讨AEE对高脂血症大鼠盲肠内容物代谢组学特征和微生物群的干预作用。结果三组大鼠分别饲喂含或不含AEE的高脂饲料（HFD）。结果表明，黄芪提取物对hfd喂养大鼠具有降低天冬氨酸转氨酶（AST）和总胆固醇（TCH）的有益作用。对照组、模型组和AEE组盲肠内容物代谢组学特征发生明显变化。hfd诱导的盲肠内容物中主要与嘌呤代谢、亚油酸代谢、甘油磷脂代谢、鞘脂代谢和嘧啶代谢相关的8种代谢物的改变被AEE处理逆转。微生物群的主坐标分析（PCoA）和聚类分析显示，AEE组与模型组的差异显著，表明AEE治疗改善了HFD对盲肠微生物群的负面影响。此外，校正分析揭示了鉴定的代谢物与盲肠微生物群之间的可能联系。结论本研究显示了AEE对盲肠内容物代谢组学特征和微生物群的调节作用，为揭示AEE治疗高脂血症的可能机制提供了信息。
BackgroundHyperlipidemia, with an increasing of prevalence, has become one of the common metabolic diseases in companion animal clinic. Aspirin eugenol ester (AEE) is a novel compound that exhibits efficacious anti-hyperlipidemia activities. However, its mechanisms are still not completely known. The objective of present study was to investigate the intervention effects of AEE on cecal contents metabonomics profile and microbiota in hyperlipidemia rats.ResultsThree groups of rats were fed with a control diet, or high fat diet (HFD) containing or not AEE. The results showed the beneficial effects of AEE in HFD-fed rats such as the reducing of aspartate aminotransferase (AST) and total cholesterol (TCH). Distinct changes in metabonomics profile of cecal contents were observed among control, model and AEE groups. HFD-induced alterations of eight metabolites in cecal contents mainly related with purine metabolism, linoleic acid metabolism, glycerophospholipid metabolism, sphingolipid metabolism and pyrimidine metabolism were reversed by AEE treatment. Principal coordinate analysis (PCoA) and cluster analysis of microbiota showed altered patterns with distinct differences in AEE group versus model group, indicating that AEE treatment improved the negative effects caused by HFD on cecal microbiota. In addition, the correction analysis revealed the possible link between the identified metabolites and cecal microbiota.ConclusionsThis study showed regulation effects of AEE on cecal contents metabonomics profile and microbiota, which could provide information to reveal the possible underlying mechanism of AEE on hyperlipidemia treatment.