Aerobic exercise of low to moderate intensity corrects unequal changes in BK(Ca) subunit expression in the mesenteric arteries of spontaneously hypertensive rats.
Aerobic exercise of low to moderate intensity corrects unequal changes in BK(Ca) subunit expression in the mesenteric arteries of spontaneously hypertensive rats.
复制标题
DOI：
10.33549/physiolres.933407
复制
发表时间：
2017-04
影响因子：
2.1
通讯作者：
Yanyan Zhang;Yu Chen;Lin Zhang;N. Lu;Lijun Shi
中科院分区：
文献类型：
--
作者：
Yanyan Zhang;Yu Chen;Lin Zhang;N. Lu;Lijun Shi
越来越多的证据表明，高血压与血管平滑肌细胞的“离子通道重构”有关。本研究旨在探讨运动强度/运动量对自发性高血压大鼠(SHR)肠系膜动脉(MA)大电导钙激活钾通道(BK(Ca))的影响。将雄性SHR随机分为3组：低强度有氧运动组(SHR-L：14m/min)、中等强度有氧运动组(SHR-M：20m/min)和久坐组(SHR)。年龄匹配的Wistar-京都大鼠(WKYS)作为正常血压对照。锻炼组完成了为期8周的锻炼计划。在SHR的MA心肌细胞中，α和β1蛋白的表达水平不同，β1亚基的增加幅度大于α亚基。与WKYS相比，BK(Ca)对SHR心肌细胞和动脉张力调节的贡献更大。与WKYS相比，SHR BK(Ca)通道亚单位蛋白表达、β1/α比值、全细胞电流密度和单通道开放概率也增加。在SHR中，有氧运动降低了全身血压，并使高血压相关的BK(Ca)变化恢复到正常血压控制水平。这些影响在中等强度组比低强度组更明显。中低强度有氧运动训练对SHR心肌细胞BK(Ca)通道的病理性适应具有纠正作用，并存在剂量效应关系。
Accumulating evidence indicates that hypertension is associated with "ion channel remodeling" of vascular smooth muscle cells (VSMCs). The objective of this study was to determine the effects of exercise intensity/volume on hypertension-associated changes in large-conductance Ca(2+)-activated K(+) (BK(Ca)) channels in mesenteric arteries (MAs) from spontaneously hypertensive rats (SHR). Male SHRs were randomly assigned to three groups: a low-intensity aerobic exercise group (SHR-L: 14 m/min), a moderate-intensity aerobic exercise group (SHR-M: 20 m/min), and a sedentary group (SHR). Age-matched Wistar-Kyoto rats (WKYs) were used as normotensive controls. Exercise groups completed an 8-week exercise program. Elevation of the alpha and beta1 proteins was unequal in MA myocytes from SHRs, with the beta1 subunit increasing more than the alpha subunit. BK(Ca) contribution to vascular tone regulation was higher in the myocytes and arteries of SHRs compared to WKYs. SHR BK(Ca) channel subunit protein expression, beta1/alpha ratio, whole cell current density and single-channel open probability was also increased compared with WKYs. Aerobic exercise lowered systemic blood pressure and normalized hypertension-associated BK(Ca) alterations to normotensive control levels in the SHRs. These effects were more pronounced in the moderate-intensity group than in the low-intensity group. There is a dose-effect for aerobic exercise training in the range of low to moderate-intensity and accompanying volume for the correction of the pathological adaptation of BK(Ca) channels in myocytes of MAs from SHR.