NEW METAL CHELATE ADSORBENT SELECTIVE FOR PROTEINS AND PEPTIDES CONTAINING NEIGHBORING HISTIDINE-RESIDUES
NEW METAL CHELATE ADSORBENT SELECTIVE FOR PROTEINS AND PEPTIDES CONTAINING NEIGHBORING HISTIDINE-RESIDUES
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DOI：
10.1016/s0021-9673(00)93969-4
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发表时间：
1987-12-18
期刊：
影响因子：
--
通讯作者：
SCHACHER, A
中科院分区：
文献类型：
--
作者：
HOCHULI, E;DOBELI, H;SCHACHER, A
制备了一种新型的氨三乙酸金属螯合亲和色谱吸附剂。这种新树脂是一种四齿螯合物形成剂，特别适合于配位数为6的金属离子，因为生物聚合物的可逆结合仍有两个化合价。已发现它对Cu 2+和Ni 2+的螯合能力强于已知的亚氨基二乙酸吸附剂，这是上级的。用Ni2+充电，评价其结合含有相邻残基的肽和蛋白质的能力。显着的特异性，使其成为一个有吸引力的除了金属螯合物亲和色谱吸附剂的范围。
A novel nitrilotriacetic acid adsorbent has been prepared for metal chelate affinity chromatography. The new resin is a quadridentate chelate former and specially suitable for metal ions with coordination numbers of six, since two valencies remain for the reversible binding of biopolymers. It has been found to chelate Cu2+ and Ni2+ strongly to be superior to the known iminodiacetic acid adsorbent. Charged with Ni2+, it was evaluated for the ability to bind peptides and proteins containing neighbouring residues. The remarkable specificity found makes it an attractive addition to the range of adsorbents for metal chelate affinity chromatography.