Glass capillary optics for producing nanometer sized beams and its applications
Glass capillary optics for producing nanometer sized beams and its applications
复制标题
用于产生纳米级光束的玻璃毛细管光学器件及其应用
DOI：
10.1016/j.surfcoat.2011.03.098
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发表时间：
2011
影响因子：
5.4
通讯作者：
T.Ikeda
中科院分区：
文献类型：
--
作者：
A. Rajendran;M. Endo;K. Hidaka;H. Sugiyaia;T.Ikeda
我们已经开发出一种方法来产生微/纳米尺寸的keV能量高电荷离子（HCIs）和MeV能量质子/He离子与锥形玻璃毛细管光学应用的表面改性和生物工具，称为“细胞手术”，分别束。用8/64 keV Ar ~（8+）离子束在入口直径为0.8mm × 10 ~（-1），出口直径从900 nm × 10 ~（-1）到几十微米，长度约50 mm的锥形玻璃毛细管中进行了透射实验。透射光束的密度增强约为10，并通过倾斜高达± 100 mrad的毛细管引导。在传输过程中保持了光束的电荷状态。MeV质子/He离子束与出口端有细窗口的毛细管相结合，可以实现精确的能量沉积和在活细胞或任何液体物体中任意位置的三维选择轰击点。用4 MeV的He ~（2+）离子注入一个端窗厚度为7.3μm、出口直径为9.6μm的毛细管中，形成微束，照射真实的生物细胞--细胞核被绿色荧光蛋白（GFP）标记的HeLa细胞。传输的MeV离子束的密度增强高达1000根据毛细管出口的大小，这是适用于各种材料分析采用微束。
We have developed a method to produce micro/nano meter sized beams of keV energy highly charged ions (HCIs) and MeV energy protons/He ions with tapered glass capillary optics for application of surface modifications and a biological tool called “cell surgery”, respectively. The transmission through the tapered glass capillaries with inlet diameter of 0.8mmϕ, outlet diameter from 900nmϕ to several tens of microns and length of about 50mm was performed using 8/64keV Ar8+beams. The transmitted beams had a density enhancement of about 10 and were guided through a capillary tilted by as large as ±100mrad. The charge state of the beams was kept during the transmission. The combination of MeV proton/He ion beams and the capillary with a thin end window at its outlet can realize pinpoint energy deposition and three-dimensional selection of the bombarding point in an arbitrary position of a living cell or in any liquid object. We demonstrated that a real biological cell, HeLa cell with the nucleus labeled by green fluorescent protein (GFP), was irradiated with the microbeam, which was prepared by 4MeV He2+entering a capillary with an end window of 7.3μm in thickness and outlet diameter of 9.6μmϕ. The transmitted MeV ion beams had density enhancement up to 1000 according to the capillary outlet sizes, which are applicable to various material analyses employing microbeams.