Response of traditional upland rice varieties to inoculation with selected diazotrophic bacteria isolated from rice cropped at the Northeast region of Brazil
Response of traditional upland rice varieties to inoculation with selected diazotrophic bacteria isolated from rice cropped at the Northeast region of Brazil
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DOI：
10.1016/j.apsoil.2012.10.004
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发表时间：
2013-02-01
影响因子：
4.8
通讯作者：
Baldani, Jose Ivo
中科院分区：
文献类型：
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作者：
da Silva Araujo, Antonio Edilson;Divan Baldani, Vera Lucia;Baldani, Jose Ivo
巴西传统旱稻品种数量最多的地区是巴西东北部地区的马拉尼昂州。然而，没有关于相关固氮细菌种群以及当这些传统基因型接种天然菌株时促进植物生长的潜力的信息。在这里，我们评估了十个传统水稻品种对接种十个固氮菌株的反应，这些菌株先前从该地区的水稻土壤中分离出来，并筛选了它们在体外产生吲哚-3-乙酸（IAA）的能力。选择最佳固氮菌株/水稻品种相互作用的程序包括三个步骤：限菌条件、土盆和田间实验。限菌实验表明，亚马逊固氮螺菌菌株 AR3122 增加了传统品种 Cana Roxa 和 Cana Forte 的生物量（分别为 28% 和 48%），而对于其他菌株/水稻品种组合，这种效果不太明显。土盆试验表明，越南伯克霍尔德氏菌菌株AR 1122与传统品种Arroz 70的组合优于其他菌株/品种以及施用100 kg N ha(-1)的处理。越南伯克霍尔德氏菌菌株 AR1122/品种 Arroz 70 的最佳性能在田间实验中得到证实。与施氮肥和Herbaspirillum seropedicae ZAE 94菌株处理相比，谷物产量分别增加了10%和29%。相比之下，商业品种Bonanca对接种菌株AR1122的反应要低得多，这表明传统水稻品种的生物肥料接种计划应考虑菌株和水稻品种之间的遗传相互作用。固氮营养 B. vietmaniensis 菌株 AR1122 是接种传统水稻品种的良好生物肥料候选者，因此应用于进一步研究，以确认菌株基因型效应，设想主要在巴西东北地区建立可持续水稻作物系统。 (C) 2012 Elsevier B.V. 保留所有权利。
The largest numbers of the Brazilian traditional upland rice varieties are found in the Maranhao state, Northeast region of Brazil. However, no information is available on the diazotrophic bacterial population associated as well as the plant growth promoting potential when these traditional genotypes are inoculated with native strains. Here, we evaluated the response of ten traditional rice varieties to inoculation with ten diazotrophic strains, previously isolated from rice soil of this region and screened for their ability to produce indole-3-acetic acid (IAA) in vitro. The procedure for selection of the best diazotrophic strain/rice variety interaction involved three steps: gnotobiotic conditions, soil pot and field experiments. The gnotobiotic experiment showed that the Azospirillum amazonense strain AR3122 increased the biomass of the traditional varieties Cana Roxa and Cana Forte (28 and 48%, respectively) while this effect was less evident for the other combination of strains/rice varieties. The soil pot experiment showed that the combination of Burkholderia vietnamiensis strain AR 1122 and traditional variety Arroz 70 was superior to the other strains/varieties and the treatment fertilized with 100 kg N ha(-1). The best performance of the Burkholderia vietnamiensis strain AR1122/variety Arroz 70 was confirmed in the field experiment. There was an increase of up 10 and 29% in the grain yield in comparison to both the N fertilization and Herbaspirillum seropedicae ZAE 94 strain treatments, respectively. In contrast, the response of the commercial variety Bonanca to inoculation with strain AR1122 was much lower, suggesting that a biofertilizer inoculation program for traditional rice varieties should consider the genetic interaction between strain and rice variety. The diazotrophic B. vietmaniensis strain AR1122 was a good biofertilizer candidate for inoculation of traditional rice varieties and therefore should be used for further studies to confirm the strain-genotype effect envisaging a sustainable rice crop system mainly in the Northeast region of Brazil. (C) 2012 Elsevier B.V. All rights reserved.