Tris(hydroxymethyl)aminomethane-functionalized silica particles and their application for hydrophilic interaction chromatography
Tris(hydroxymethyl)aminomethane-functionalized silica particles and their application for hydrophilic interaction chromatography
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DOI：
10.1002/jssc.201000154
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发表时间：
2010-09-01
影响因子：
3.1
通讯作者：
Irgum, Knut
中科院分区：
文献类型：
--
作者：
Bui, Nhat Thi Hong;Verhage, Jeroen J.;Irgum, Knut
提出了一种新的合成高亲水性硅胶材料的方法。用3-溴丙基三氯硅烷对用作起始基质的多孔二氧化硅颗粒进行改性，并通过受控（“活性”）原子转移自由基聚合用甲基丙烯酸缩水甘油酯接枝，以在二氧化硅表面上引入携带环氧乙烷的反应性表面活性剂层。然后，使接枝材料与三（羟甲基）氨基甲烷在二甲基甲酰胺中进行环氧乙烷开环反应，以产生公知的和高度亲水的“TRIS”缓冲物质的聚合物结合的等价物。通过漫反射FT-IR、X射线光电子能谱、元素分析和H-1 NMR进行化学表征。使用Brunauer-Emmett-Teller进行孔隙率和表面积检查。通过在混合亲水相互作用色谱和弱阴离子交换条件下分离核酸碱基、小有机酸和常见核苷酸来评价该材料的色谱应用。
A new method is presented for synthesizing a highly hydrophilic silica-based material for use in hydrophilic interaction chromatography. Porous silica particles used as a starting substrate were modified with 3-bromopropyl trichlorosilane and grafted with glycidyl methacrylate by controlled ("living") atom transfer radical polymerization in order to introduce an oxirane-carrying reactive tentacle layer on the silica surface. The grafted material was thereafter subject to an oxirane ring opening reaction with tris(hydroxymethyl)aminomethane in dimethylformamide to yield a polymer-bound equivalent of the well known and highly hydrophilic "TRIS" buffering substance. Chemical characterization was done by diffuse reflectance FT-IR, X-ray photoelectron spectroscopy, elemental analysis, and H-1 NMR. Porosity and surface area examination was done with Brunauer-Emmett-Teller. Chromatographic application of the material was evaluated by separations of nucleic bases, small organic acids, and common nucleotides under mixed hydrophilic interaction chromatography and weak anion exchange conditions.