Ultrastructural localization of alpha-keratins in the regenerating epidermis of the lizard Podarcis muralis during formation of the shedding layer
Ultrastructural localization of alpha-keratins in the regenerating epidermis of the lizard Podarcis muralis during formation of the shedding layer
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DOI：
10.1054/tice.2000.0099
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发表时间：
2000-04-01
期刊：
影响因子：
2.6
通讯作者：
Alibardi, L
中科院分区：
文献类型：
--
作者：
Alibardi, L
在蜥蜴的表皮中，α-和β-角蛋白在脱落周期期间依次产生。利用包埋前后的免疫细胞化学，本研究显示了3种α-角蛋白抗体的超微结构分布识别酸性低MW角蛋白的AE 1抗体局限于基底和基底上细胞中的张力丝束，但不存在于角化β-和α-细胞中，识别较高MW角蛋白的AE 2抗体弱染色预角化细胞和强烈角化的α层。弱标记存在于β层内的小电子致密区域中。识别低和高MW碱性角蛋白的AE 3抗体可免疫所有表皮层中的张力细丝束，但强烈地免疫α-角化和角化层（中间层、α-角化层、腔隙层和透明层）。角质透明蛋白样颗粒存在于脱落层的透明细胞中，对这些抗体呈阴性，因此角质透明层含有与非角蛋白物质混合的角蛋白。AE 3抗体显示，成熟的β-层和上郝琴的棘状褶皱仅在普遍的电子-苍白的β-角蛋白材料中的小的致密区域中被标记。因此，一些α-角蛋白仍然存在于β-层中，并支持α-角蛋白（碱性）作为β-角蛋白沉积的支架的想法。(C)2000年哈考特出版社有限公司
In the epidermis of lizards, alpha- and beta-keratins are sequentially produced during a shedding cycle. Using pre- and post-embedding immunocytochemistry this study shows the ultrastructural distribution of 3 alpha-keratin antibodies (AE1, AE2, AE3) in the renewing epidermis and in the shedding complex of the regenerating tail of the lizard Podarcis muralis, The AE1 antibody that recognizes acidic low MW keratins is confined to tonofilament bundles in basal and suprabasal cells but is not present in keratinizing beta- and alpha-cells, The AE2 antibody that recognises higher MW keratins weakly stains pre-keratinized cells and intensely keratinized alpha-layers, A weak labeling is present in small electrondense areas within the beta-layer. The AE3 antibody, that recognizes low and high MW basic keratins, immunolables tonofilament bundles in all epidermal layers but intensely the alpha-keratinizing and keratinized layers (mesos, alpha-, lacunar and clear). Keratohyalin-like granules, present in the clear cells of the shedding layer, are negative to these antibodies so that the cornified clear layer contains keratins mixed with non-keratin material. The AE3 antibody shows that the mature beta-layer and the spinulated folds of the oberhautchen are labeled only in small dense areas among the prevalent electron-pale beta-keratin material. Therefore, some alpha-keratin is still present in the beta-layer, and supports the idea that alpha-keratins (basic) function as scaffold for beta-keratin deposition. (C) 2000 Harcourt Publishers Ltd.