Estimation of N or C uptake rates by phytoplankton using 15N or 13C: revisiting the usual computation formulae
Estimation of N or C uptake rates by phytoplankton using 15N or 13C: revisiting the usual computation formulae
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使用 15N 或 13C 估算浮游植物的 N 或 C 吸收率：重新审视常用的计算公式
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Michel Gosselin
中科院分区：
文献类型：
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作者：
L. Legendre;Michel Gosselin
浮游植物对 N 的吸收通常使用 I5N 技术估算，在某些情况下，C 的吸收则使用 13 C 估算。对净运输率计算公式的严格检查得出了一些有趣甚至意想不到的结论。这些是用于计算净传输速率 (p) 的 I5N 或 BC 技术公式与 14C 技术的公式相同，尽管存在明显的差异，这反映了用于确定非放射性和放射性同位素的设备的差异；所谓的比吸收率 (V) 不应用于自然样品，除非作为计算传输率 (p) 的一个步骤； p 的估计不受培养样品中是否存在非浮游植物圆锥状有机物 (POM) 的影响；应停止在孵育开始时将示踪剂浓度添加到表示溶解相中示踪剂浓度的表达式分母中的做法； POM 的浓度应根据培养结束时的接种样品（或者平行培养的样品）确定，而不是根据培养开始时采集的水样确定。
The uptake of N by phytoplankton is generally estimated using the I5N technique and, under some circumstances, the uptake of C is estimated using 13C. Rigorous examination of formulae for computing net transport rates leads to several interesting and even unexpected conclusions. These are that the I5N or BC technique formula for computing net transport rates (p) is identical to that of the 14C technique, in spite of apparent dissimilarities which reflect differences in equipment used for determining non-radioactive and radioactive isotopes; the so-called specific uptake rates (V) should not be used with natural samples, except as a step in the calculation of transport rates (p); estimation of p is unaffected by the presence/absence of non-phytoplanktonic paniculate organic matter (POM) in the incubated sample; the practice of adding the concentration of tracer to the denominator of expression representing the concentration of tracer in the dissolved phase at the beginning of incuba- tion should be discontinued; and the concentration of POM should be determined from the inocu- lated sample at the end of incubation (or, alternatively, from a sample incubated in parallel) and not from a water sample taken at the beginning of the incubation.