Circularization of mRNA by eukaryotic translation initiation factors
Circularization of mRNA by eukaryotic translation initiation factors
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DOI：
10.1016/s1097-2765(00)80122-7
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发表时间：
1998-07-01
期刊：
影响因子：
16
通讯作者：
Sachs, AB
中科院分区：
文献类型：
--
作者：
Wells, SE;Hillner, PE;Sachs, AB
真核 mRNA 的 5'帽结构和 3'poly(A) 尾之间的通讯导致翻译的协同增强。帽和多聚腺苷酸尾结合蛋白 eF4E 和 Pab1p 通过与翻译因子 eF4G 的不同部分相互作用，在酿酒酵母中介导这种效应。在这里，我们展示了用重组蛋白重建 elF4E/elF4G/Pab1p 复合物，并通过原子力显微镜显示该复合物可以环化加帽的聚腺苷酸化 RNA。我们的结果表明，翻译因子形成的环状 mRNA 可能有助于控制真核细胞中 mRNA 的表达。
Communication between the 5' cap structure and 3' poly(A) tail of eukaryotic mRNA results in the synergistic enhancement of translation. The cap and poly(A) tail binding proteins, elF4E and Pab1p, mediate this effect in the yeast S. cerevisiae through their interactions with different parts of the translation factor elF4G. Here, we demonstrate the reconstitution of an elF4E/elF4G/Pab1p complex with recombinant proteins, and show by atomic force microscopy that the complex can circularize capped, polyadenylated RNA. Our results suggest that formation of circular mRNA by translation factors could contribute to the control of mRNA expression in the eukaryotic cell.