Organization of the simian hemorrhagic fever virus genome and identification of the sgRNA junction sequences.
Organization of the simian hemorrhagic fever virus genome and identification of the sgRNA junction sequences.
复制标题
猿猴出血热病毒基因组的组织和 sgRNA 连接序列的鉴定。
DOI：
10.1007/978-1-4615-5331-1_37
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发表时间：
1998
影响因子：
--
通讯作者：
Godeny,EK
中科院分区：
文献类型：
--
作者：
Wang,XC;Smith,SL;Godeny,EK
SHFV是动脉病毒科的一员。该家族中的病毒编码八个开放阅读框架(ORF)，其中两个是从全长基因组RNA翻译而来的。剩下的6个ORF是从6个或7个3‘端共末端亚基因组RNA(SgRNAs)的嵌套集合翻译而来的。我们已经从SHFV基因组的3‘端克隆并测序了大约6000个核苷酸(NT)。共鉴定出11个开放阅读框，编号分别为1a、1b、2a、2b、3、4、5、6、7、8和9，比其他动脉病毒多3个。由ORF2a到ORF9编码的多肽的特征是从它们的计算机生成的氨基酸序列确定的。我们还利用RT-PCR技术从SHFV的每个亚基因组RNA(SgRNAs)中扩增出连接序列。发现了8个独立的连接序列，这表明SHFV在复制过程中产生8个sgRNAs。ORF 2a和2b似乎编码在相同的sgRNA上，这意味着RNA2是多顺反子的。序列分析表明，SHFV基因的保守序列为5‘-(U/C)(C/U)N(U/C)(U/C)(A/C/G)AC(C/U)-3’。由于SHFV编码额外的ORF并在复制过程中产生额外的sgRNAs，这些数据表明SHFV可能比其他动脉病毒更复杂。
SHFV is a member of theArteriviridaefamily. Viruses within this family encode eight open reading frames (ORFs), two of which are translated from the full-length genome RNA. The remaining six ORFs are translated from a nested set of six or seven 3’ co-terminal, subgenomic RNAs (sgRNAs). We have cloned and sequenced approximately 6000 nucleotides (nt) from the 3’ end of the SHFV genome. Eleven ORFs, numbered ORFs 1a, 1b, 2a, 2b, 3, 4, 5, 6, 7, 8, and 9, were identified, three more than the other arteriviruses. The characteristics of the peptides encoded by ORFs 2a through 9 were determined from their computer-generated amino acid sequences. We also amplified the junction sequences from each of the SHFV subgenomic RNAs (sgRNAs) using RT-PCR analysis. Eight separate junction sequences were found which suggests that SHFV produces eight sgRNAs during replication. ORFs 2a and 2b appear to be encoded on the same sgRNA implying that RNA 2 is polycistronic. Sequence analysis identified the conserved SHFV junction sequence as 5’-(U/C)(C/U)N(U/C)(U/C)(A/C/G)AC(C/U)-3’. Since SHFV encodes additional ORFs and produces additional sgRNAs during replication, these data suggest that SHFV may be more complex than the other arteriviruses.