The genomic sequence and comparative analysis of the rat major histocompatibility complex
The genomic sequence and comparative analysis of the rat major histocompatibility complex
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DOI：
10.1101/gr.1987704
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发表时间：
2004-04-01
期刊：
影响因子：
7
通讯作者：
Himmelbauer, H
中科院分区：
文献类型：
--
作者：
Hurt, P;Walter, L;Himmelbauer, H
我们已经确定了大鼠染色体20p12上包含大鼠主要组织相容性复合体(MHC)的4-Mb区间的序列。这是首次报道大鼠基因组片段的完整序列，并构成了迄今为止啮齿动物基因组中最大的连续序列之一。老鼠MHC是仅次于人类MHC的第二个完成测序的哺乳动物MHC。我们的分析已经确定了位于测序区间内的至少220个基因。虽然在第II类和第III类基因区间以及骨架基因区域中，基因的含量和顺序都很保守，但与人类和小鼠相比，在第I类基因区域中发现了深刻的大鼠特有的特征。在I类基因和基因家族的结构水平、数量和组织上都发现了与I类区域相关的差异，在更全球化的背景下，在进化塑造当今大鼠MHC的方式上也发现了差异。
We have determined the sequence of a 4-Mb interval on rat chromosome 20p12 that encompasses the rat major histocompatibility complex (MHC). This is the first report of a finished sequence for a segment of the rat genome and constitutes one of the largest contiguous sequences thus far for rodent genomes in general. The rat MHC is, next to the human MHC, the second mammalian MHC sequenced to completion. Our analysis has resulted in the identification of at least 220 genes located within the sequenced interval. Although gene content and order are well conserved in the class II and class III gene intervals as well as the framework gene regions, profound rat-specific features were encountered within the class I gene regions, in comparison to human and mouse. Class I region-associated differences were found both at the structural level, the number, and organization of class I genes and gene families, and, in a more global context, in the way that evolution worked to shape the present-day rat MHC.