Genetic diversity of Actinidia arguta in Northern-East China as revealed by RAPD polymorphism.
Genetic diversity of Actinidia arguta in Northern-East China as revealed by RAPD polymorphism.
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DOI：
10.1145/1943552.1943575
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Zhao, X.
中科院分区：
文献类型：
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作者：
Dai, H.;Liu, C.;Zhao, X.
软枣猕猴桃（Actinidia arguta (Sieb. & Zucc.) Planch. ex Miq.）原产于中国北方。收集了来自9个地区的32份软枣猕猴桃材料，利用随机扩增多态性DNA（RAPD）标记评估其遗传多样性。在120个RAPD引物中，选取18个能产生清晰且信息丰富条带的引物对所有材料进行基因分型。在126个清晰且可重复的片段中，114个（每个引物6.3个）具有多态性。根据RAPD数据，采用算术平均非加权成组配对法（UPGMA）构建聚类图。基于RAPD标记的差异系数在0.15到0.58之间。差异系数的范围表明软枣猕猴桃具有较高的遗传多样性。来自吉林省临江市的9份材料的总片段数为123个，这9份材料之间的差异系数在0.15到0.56之间，这表明临江市的软枣猕猴桃种质具有丰富的遗传多样性。
Actinidia arguta (Sieb. & Zucc.) Planch. ex Miq. is native to northern China. A collection of 32 accessions of A. arguta from nine regions was used to evaluate genetic diversity with random amplified polymorphic DNA (RAPD) markers. Of the 120 RAPD primers, the 18 that proved to generate clear and highly informative profiles were used to genotype the full panel of accessions. Among the 126 clear and reproducible fragments, 114 (6.3 per primer) were polymorphic. Dendrograms based on unweighted pair group method with arithmetic average analysis were constructed from the RAPD data. Difference coefficients based on RAPD markers ranged from 0.15 to 0.58. The range in difference coefficients indicated that A. arguta has a high level of genetic diversity. The total number of fragments across nine accessions that were collected from Linjiang region in Jilin province was 123, and the difference coefficients between the nine accessions ranged from 0.15 to 0.56. It indicates that the A. arguta germplasm in Linjiang region contains rich genetic diversity.