Evidence for the local occurrence of angiotensin II in rat kidney and its modulation by dietary sodium intake and converting enzyme blockade.
Evidence for the local occurrence of angiotensin II in rat kidney and its modulation by dietary sodium intake and converting enzyme blockade.
复制标题
血管紧张素 II 在大鼠肾脏中局部存在及其通过膳食钠摄入和转化酶阻断进行调节的证据。
DOI：
10.1042/cs0570173
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发表时间：
1979
期刊：
影响因子：
6
通讯作者：
F. Mendelsohn
中科院分区：
文献类型：
--
作者：
F. Mendelsohn
1.用离子交换法和免疫吸附法纯化血管紧张素Ⅱ（ANG Ⅱ）肽，然后用放射免疫分析法测定了快速冷冻大鼠肾的酸性乙醇匀浆中的ANG Ⅱ。2.肾组织中ANG Ⅱ浓度是血浆的10--20倍。3.用等渗氯化钠溶液或EDTA二钠溶液经肾动脉灌注肾脏不会改变肾内ANG II的浓度。4.动物喂养的钠缺乏饮食8天有显着较高浓度的肾内血管紧张素II，血浆肾素活性和肾脏肾素浓度比钠充足的动物。5.口服钠负荷3周后，大鼠血浆肾素活性和肾脏肾素浓度受到抑制，但与正常钠摄入量的动物相比，肾内ANG II无变化。6.在给予转化酶抑制剂（SQ 20881）后1小时，血浆肾素活性升高，肾脏肾素浓度不变，肾内ANG II被抑制。7.这些结果表明ANG II存在于肾脏的血管外隔室中。他们进一步表明，其数量受钠摄入量的影响，血管紧张素I转化酶对其形成至关重要。
1. Angiotensin II (ANG II) was measured in acid-ethanol homogenates of rapidly frozen rat kidneys by a method involving ion-exchange and immunoadsorbent purification of peptides before radioimmunoassay. 2. Concentrations of ANG II found in kidney were 10--20 times that in plasma. 3. Perfusion of the kidneys via the renal artery with isotonic sodium chloride solution or with disodium EDTA solution did not alter the concentrations of intrarenal ANG II. 4. Animals fed on a sodium-deficient diet for 8 days had markedly higher concentrations of intrarenal ANG II, plasma renin activity and kidney renin concentration than sodium-replete animals. 5. After oral sodium loading for 3 weeks, rats had suppressed plasma renin activity and kidney renin concentration but unchanged intrarenal ANG II when compared with animals on a normal sodium intake. 6. One hour after the administration of a converting enzyme inhibitor (SQ 20881) plasma renin activity was elevated, kidney renin concentration unchanged and intrarenal ANG II was depressed. 7. These results demonstrate the presence of ANG II in the extravascular compartment of the kidney. They further suggest that its quantity is influenced by sodium intake and that angiotensin I converting enzyme is essential for its formation.