High-efficiency capillary isoelectric focusing of peptides
High-efficiency capillary isoelectric focusing of peptides
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DOI：
10.1021/ac991367t
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发表时间：
2000-05-01
影响因子：
7.4
通讯作者：
Smith, RD
中科院分区：
文献类型：
--
作者：
Shen, YF;Berger, SJ;Smith, RD
目前正在开发的几种方法，全球蛋白质组表征的基础上，从消化（往往是复杂的）蛋白质混合物的多肽混合物的分析。需要用于肽分析的改进方法，其提供样品浓缩、更高分辨率分离以及与质谱法的直接兼容性。在这项工作中，已经开发了用于肽的高效毛细管等电聚焦（CIEF）分离的方法，该方法根据肽的等电点提供同时的样品浓缩和分离。在典型的非变性CIEF条件下，肽被浓缩至类似于500倍，并且肽以约100倍存在。
Several approaches are presently being developed for global proteome characterization that are based upon the analysis of polypeptide mixtures resulting from digestion of (often complex) mixtures of proteins. Improved methods for,peptide analysis are needed that provide for sample concentration, higher resolution separations, and direct compatibility with mass spectrometry. In this work, methods for the high-efficiency capillary isoelectric focusing (CIEF) separation of peptides have been developed that provide for simultaneous sample concentration and separation according to peptide isoelectric point. Under typical nondenaturing CIEF conditions, peptides are concentrated similar to 500-fold, and peptides present at