Delta-9-tetrahydrocannabinol (THC), the major psychoactive component of marijuana, exacerbates brain infection by Acanthamoeba.
Delta-9-tetrahydrocannabinol (THC), the major psychoactive component of marijuana, exacerbates brain infection by Acanthamoeba.
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Delta-9-四氢大麻酚 (THC) 是大麻的主要精神活性成分，会加剧棘阿米巴的脑部感染。
DOI：
10.1111/j.1550-7408.2001.tb00433.x
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Cabral,G
中科院分区：
文献类型：
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作者：
Marciano-Cabral,F;Ferguson,T;Bradley,SG;Cabral,G
材料和方法将卡氏棘阿米巴 (ATCC 30010) 和库伯氏棘阿米巴 (ATCC 30171) 维持在 37°C 的补充有小牛血清和氯化血红素的 Oxoid 培养基中。通过用汉克斯平衡盐溶液(HBSS)洗涤来收获阿米巴，使用血细胞计数器进行计数，并调整至A.castellanii的3×10 6/10μl和A.culbertsoni的1×10 3/10μl。使用第二种卡斯特拉尼菌株（ATCC 50494）来检查 THC 处理和未处理小鼠中对棘阿米巴的抗体反应，因为该菌株在连续无菌培养后失去了毒力。使用后一种菌株可以使小鼠存活并评估免疫反应性，而不会因动物死亡而受到影响。
MATERIALS AND METHODSAcanthamoeba castellanii (ATCC 30010) and Acanthamoeba culbertsoni (ATCC 30171) were maintained at 37 C in Oxoid medium supplemented with calf serum and hemin. Amebae were harvested by washing with Hanks' Balanced Salt Solution (HBSS), counted using a hemacytometer, and adjusted to 3× 10 6/10 μl for A. castellanii and 1× 10 3/10 μl for A. culbertsoni. A second strain of A. castellanii,(ATCC 50494), was used to examine the antibody response to Acanthamoeba in THC-treated and untreated mice because this strain lost its virulence after continuous axenic culture. Use of this latter strain allowed for survival of mice and assessment of immune responsiveness uncompromised by animal mortalities.