A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles
A simple and rapid detection system for oral bacteria in liquid phase for point-of-care diagnostics using magnetic nanoparticles
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DOI：
10.1063/1.5130437
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发表时间：
2019-12
期刊：
影响因子：
1.6
通讯作者：
L. Tonthat;S. Takahashi;Hidehiko Onodera;K. Okita;S. Yabukami;Kotone Yokota;Maiko Furuya;H. Kanetaka;Y. Miura;Hideki Takahashi;Yoshihiko Watanabe;R. Akiyama
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文献类型：
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作者：
L. Tonthat;S. Takahashi;Hidehiko Onodera;K. Okita;S. Yabukami;Kotone Yokota;Maiko Furuya;H. Kanetaka;Y. Miura;Hideki Takahashi;Yoshihiko Watanabe;R. Akiyama
本研究描述了一种用户友好且快速的液相口腔细菌检测系统，用于基于磁免疫分析的护理点测试。我们重点研究了切换与细菌结合的纳米颗粒的磁矩所需的外部磁场强度对细菌浓度的依赖性。获得的结果表明，所需的场强随着在 103-109 CFU/mL 范围内培养的牙龈卟啉单胞菌浓度的对数线性增加。类似地，当变形链球菌和铜绿假单胞菌的浓度增加时，所需的场强单调增加，而大肠杆菌的所需场强当其浓度增加时单调减少。然后，我们测量了老年保健服务机构中从老年人身上收集的唾液中牙龈卟啉单胞菌的浓度，使用开发的系统得到的结果与使用商业细菌计数器得到的结果具有相关性。
This study describes a user-friendly and rapid detection system of oral bacteria in the liquid phase for point of care testing based on magnetic immunoassay. We focused on the dependence of the strength of external magnetic field required to switch the magnetic moments of nanoparticles bound to bacteria on the bacteria concentration. The results obtained indicate that the required field strength increases linearly as a function of log concentration of Porphyromonas gingivalis cultured in the range of 103–109 CFU/mL. Similarly, the required field strengths for Streptococcus mutans and Pseudomonas aeruginosa increase monotonically when their concentrations increase, whereas the required field strength for Escherichia coli decreases monotonically when its concentration increases. We then measured the concentration of Porphyromonas gingivalis in the saliva collected from elderly people in a geriatric health services facility and the results using the developed system had a correlation with those using a commercial bacteria counter.