Molecular identification of Tetrahymena species.
Molecular identification of Tetrahymena species.
复制标题
四膜虫物种的分子鉴定。
DOI：
10.1111/jeu.12936
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Clark,Theodore
中科院分区：
文献类型：
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作者：
Cassidy-Hanley,DonnaMarie;Doerder,FPaul;Hossain,Mozammal;Devine,Catherine;Clark,Theodore
线粒体基因组1689 bp的条形码常用于四膜虫种的鉴定。在这里，我们研究是否两个较短的核序列，5.8S rRNA基因区域和H3和H4组蛋白基因之间的基因间区域，也可能是有用的，无论是单独或相互结合orcox 1。我们获得了保存在四膜虫库存中心的约300个野生分离株的序列，并分析了从基因库获得的其他序列。5.8S rRNA基因及其转录侧翼的部分鉴定了分离株的主要进化枝，并独特地鉴定了一些但不是全部的物种。约330 bp的H3/H4基因间区具有较低的种内变异性，是大多数物种所特有的。然而，它无法区分两对常见物种和它们的稀有物种，而且它的使用因某些物种中存在重复基因而变得复杂。结果表明，虽然cox 1序列是四膜虫种鉴定的最佳单一标记，但5.8S rRNA和H3/H4基因间区序列可单独或组合用于确认cox 1种分配或作为新采集的四膜虫初步调查的一部分。从我们新收集的分离物中，结果扩大了上海四膜虫和马拉重四膜虫的地理范围，并鉴定了一个新种--阿氏四膜虫。sp.描述的。
Mitochondrialcox1 689 bp barcodes are routinely used for identification ofTetrahymenaspecies. Here, we examine whether two shorter nuclear sequences, the 5.8S rRNA gene region and the intergenic region between H3 and H4 histone genes, might also be useful either singly or in combination with each other orcox1. We obtained sequences from ~300 wild isolates deposited at the Tetrahymena Stock Center and analyzed additional sequences obtained from GenBank. The 5.8S rRNA gene and portions of its transcribed flanks identify isolates as to their major clade and uniquely identify some, but not all, species. The ~330 bp H3/H4 intergenic region possesses low intraspecific variability and is unique for most species. However, it fails to distinguish between two pairs of common species and their rarer counterparts, and its use is complicated by the presence of duplicate genes in some species. The results show that while thecox1 sequence is the best single marker forTetrahymenaspecies identification, 5.8S rRNA, and the H3/H4 intergenic regions sequences are useful, singly or in combination, to confirmcox1 species assignments or as part of a preliminary survey of newly collectedTetrahymena. From our newly collected isolates, the results extend the biogeographical range ofT.shanghaiensisandT.malaccensisand identify a new species,Tetrahymena arleneaen. sp. herein described.