EFFECT OF HYPOXIA ON ENERGY AND CALCUM METABOLISM IN AIRWAY SMOOTH MUSCLE
EFFECT OF HYPOXIA ON ENERGY AND CALCUM METABOLISM IN AIRWAY SMOOTH MUSCLE
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DOI：
10.1152/ajplegacy.1971.220.5.1199
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发表时间：
1971-01-01
影响因子：
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通讯作者：
STEPHENS, NL
中科院分区：
文献类型：
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作者：
KROEGER, E;STEPHENS, NL
方法应用戊巴比妥30 mg/kg麻醉后，取杂种犬颈气管气管组织取棘肌。如前所述，解剖肌条并在Krebs-Ringer溶液中平衡（33）。用02-CO2气体混合物平衡溶液，在温度为37℃和1℃时，测得镀液Paz为600 mm Hg， Pcoz为45 mm Hg， pH为7.40。还制备了N2-CO2混合物，使镀液Paz在保持pH和Pcoz恒定的情况下迅速降至< 10 mm Hg。
METHODSTrachealis muscle was obtained from the cervical tracheas of mongrel dogs anesthetized with 30 mg/kg pentobarbital. Muscle strips were dissected and equilibrated in a Krebs-Ringer solution as described previously(33). The solution was equilibrated with an 02-CO2 gas mixture so as to achieve a measured bath Paz of 600 mm Hg, Pcoz of 45 mm Hg, and pH of 7.40 at a temperature of 37 & 1 C. A N2-CO2 mixture was also prepared so that the bath Paz could be rapidly decreased to< 10 mm Hg while maintaining pH and PCOZ constant.