Effect of Nd3+ ion on carboxylation activity of ribulose-1,5-bisphosphate carboxylase/oxygenase of spinach.
Effect of Nd3+ ion on carboxylation activity of ribulose-1,5-bisphosphate carboxylase/oxygenase of spinach.
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DOI：
10.1016/j.bbrc.2006.01.105
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发表时间：
2006-03
影响因子：
3.1
通讯作者：
Chao Liu;Fa-shui Hong;Kang Wu;Hong-bing Ma;Xue-guang Zhang;Cheng-jiao Hong;Cheng Wu;Fengqing Gao;Fan Yang;Lei Zheng;Xuefeng Wang;Taozhe Liu;Ya‐ning Xie;Jianhua Xu;Zhong-rui Li
中科院分区：
文献类型：
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作者：
Chao Liu;Fa-shui Hong;Kang Wu;Hong-bing Ma;Xue-guang Zhang;Cheng-jiao Hong;Cheng Wu;Fengqing Gao;Fan Yang;Lei Zheng;Xuefeng Wang;Taozhe Liu;Ya‐ning Xie;Jianhua Xu;Zhong-rui Li
稀土元素钕（Nd）通过提高Rubisco羧化活性，促进菠菜光合速率和有机物质积累。Nd 3+处理对菠菜Rubisco的加氧酶活性影响不大，但使Rubisco的比因子大大提高。这部分是由于Rubisco活化酶（R-A）活性的提高，但主要是由于Rubisco-Rubisco活化酶超复合物的形成，即一种分子量较大的蛋白质（约1200 kD），它同时含有Rubisco和Rubisco活化酶。通过凝胶电泳和Western-blot研究，在Rubisco的提取过程中发现了这种超复合物。Rubisco-R-A超复合物的形成表明，从Nd ~（3+）处理的菠菜中纯化的蛋白质的二级结构不同于对照。扩展X-射线吸收精细结构研究表明，Nd与氨基酸残基中的4个氧原子和2个硫原子结合，其Nd-O键长为2.46 nm，Nd-S键长为2.89 nm。
Neodymium (Nd), as a member of rare earth elements, proved to enhance the photosynthesis rate and organic substance accumulation of spinach through the increase in carboxylation activity of Rubisco. Although the oxygenase activity of spinach Rubisco was slightly changed with the Nd3+treatment, the specific factor of Rubisco was greatly increased. It was partially due to the promotion of Rubisco activase (R-A) activity but mainly to the formation of Rubisco–Rubisco activase super-complex, a heavier molecular mass protein (about 1200kD) comprising both Rubisco and Rubisco activase. This super-complex was found during the extraction procedure of Rubisco by the gel electrophoresis and Western-blot studies. The formation of Rubisco–R-A super-complex suggested that the secondary structure of the protein purified from the Nd3+-treated spinach was different from that of the control. Extended X-ray absorption fine structure study of the ‘Rubisco’ purified from the Nd3+-treated spinach revealed that Nd was bound with four oxygen atoms and two sulfur atoms of amino acid residues at the Nd–O and Nd–S bond lengths of 2.46 and 2.89Å, respectively.