Validation of the anti-bacteremic efficacy of an essential oil rinse in a Brazilian population: a cross-over study
Validation of the anti-bacteremic efficacy of an essential oil rinse in a Brazilian population: a cross-over study
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DOI：
10.1590/s1806-83242012005000021
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发表时间：
2012-10-01
影响因子：
2.5
通讯作者：
Araujo, Marcelo Werneck Barata de
中科院分区：
文献类型：
--
作者：
Cortelli, José Roberto;Cogo, Karina;Araujo, Marcelo Werneck Barata de
这项交叉研究旨在通过测定与诱发菌血症相关的微生物的血液水平并调查巴西轻度至中度牙龈炎患者中该事件的患病率来评估精油漱口液（EOM）的杀菌功效。在109名筛选的受试者（50名男性和59名女性）中，有34名（31.19%）受试者因咀嚼定量苹果而诱发菌血症。将损伤前和损伤后血样之间至少10个菌落形成单位的差异定义为阳性结果。在接下来的两周里，患者经历了牙刷加氟化物的正常化阶段，然后安排了如下的I期方案。在基线I，受试者被指示咀嚼新的苹果配给，在这种口腔刺激前后采集新的血液样本，并随机分配到实验精油（n = 17）或安慰剂（P）漱口水（n = 17）治疗2周。在第一阶段结束时重复这些程序，然后进行为期两周的洗脱期（用氟化物刷牙）。在基线和II期结束时，当受试者交叉至其他EOM组或安慰剂组时，再次诱导菌血症。通过协方差分析评估基线和治疗后2周（EOM vs P）采集的血液样本中细菌计数的差异。EOM治疗后，平均需氧菌计数下降了45.8%，而平均无氧菌计数下降了63.3%。施磷后，好氧菌增加了28.4%，厌氧菌减少了18.5%。这项研究验证了这种新的方法，并表明EOM的杀菌作用显着减少菌血症。
This cross-over study was conducted to assess the germ-killing efficacy of an essential oil mouthrinse (EOM) by determining the blood levels of microorganisms associated with induced bacteremia and investigating the prevalence of this event in Brazilians with mild-to-moderate gingivitis. Thirty four (31.19%) subjects positive for bacteremia induced by chewing a ration of apple were enrolled out of 109 screened subjects (50 males and 59 females). A difference of at least 10 colony forming units between the pre- and post-insult blood samples was defined as a positive result. For the following two weeks patients underwent a toothbrush plus fluoride dentifrice normalization period, and were then scheduled for the Phase I protocol as follows. At baseline I, subjects were instructed to chew a new apple ration, had new blood samples taken before and after this oral stimulus, and were randomly assigned to an experimental essential oil (n = 17) or placebo (P) mouthrinse (n = 17) treatment for 2 weeks. These procedures were repeated at the end of Phase I and then followed by a two-week wash-out period (tooth brushing with fluoride dentifrice). Bacteremia was again induced at baseline and at the end of Phase II, when subjects were crossed-over to the other EOM or placebo groups. Bacterial count differences between baseline and 2-week post-treatment (EOM versus P) in the blood samples collected were assessed by analysis of covariance. Mean aerobic counts decreased by 45.8%, whereas mean anaerobic counts decreased by 63.3% after EOM treatment. After the P treatment, aerobic bacteria increased by 28.4% and anaerobic bacteria decreased by 18.5%. This study validated this novel methodology and showed that the germ-killing action of EOM significantly reduced bacteremia.