Efficacy of fenugreek plant for ascorbic acid assisted phytoextraction of copper (Cu); A detailed study of Cu induced morpho-physiological and biochemical alterations
Efficacy of fenugreek plant for ascorbic acid assisted phytoextraction of copper (Cu); A detailed study of Cu induced morpho-physiological and biochemical alterations
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DOI：
10.1016/j.chemosphere.2020.126424
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发表时间：
2020-07-01
期刊：
影响因子：
8.8
通讯作者：
Ali, Shafaqat
中科院分区：
文献类型：
--
作者：
Fatima, Arooj;Farid, Mujahid;Ali, Shafaqat
植物萃取修复是公认的经济、可持续的重金属去除方法。本研究的双重目的是研究胡芦巴在抗坏血酸（AA）影响下对铜的修复潜力。采用完全随机设计（CRD）方法，研究了铜-抗坏血酸螯合对葫芦巴生长调节及其富集铜的影响。采用AA （5 mM）和Cu（100、250和500 μ M）处理葫芦巴幼苗。铜胁迫显著降低了葫芦巴植株的生长、生物量和叶绿素等形态生理参数。而随着Cu浓度的增加，抗氧化酶活性、电解质泄漏活性和活性氧活性均增强。结果表明，与对照和纯Cu处理相比，AA介导的葫芦巴植株的生长、生物量、生理和抗氧化酶显著增加，活性氧和电解质产量显著降低。结果表明，与纯Cu处理相比，AA处理可使叶片中Cu含量提高42%，茎中Cu含量提高18%，根部Cu含量提高45%。此外，AA的应用表明研究结果揭示了其在Cu胁迫下的生长调节剂和螯合剂的作用。(C) 2020 Elsevier Ltd.版权所有。
Phytoremediation via phyto-extraction is well recognized and sustainable principle for the economical removal of heavy metals from contaminated water and soil. The twofold objective of the present research work was to investigate the remediation potential of fenugreek for Cu under the influence of ascorbic acid (AA). The effect of copper-ascorbic acid chelation on the growth regulation of fenugreek (Trigonella foenum-graceum L.) and its potential to accumulate Cu was investigated in hydroponic medium to optimize concentration with complete randomized design (CRD). Juvenile fenugreek plants were treated with different treatments of AA (5 mM) and Cu (100, 250 and 500 mu M). The different morphophysiological parameters of fenugreek plant such as growth, biomass and chlorophylls were significantly reduced under Cu stress. However, the activities of antioxidant enzymes, electrolyte leakage and reactive oxygen species enhanced with increasing concentration of applied Cu. Results indicated significant increase in plant growth, biomass, physiology and antioxidant enzymes and decrease in reactive oxygen species and electrolyte production in AA mediated fenugreek plants compared to controls and Cu only treated plants. However, it was also found that AA enhanced Cu concentration maximum up to 42% in leaf, 18% in stem and 45% in roots as compared to Cu treated only plants. Moreover, application of AA signified the research results revealing to act as growth regulator and chelator under Cu stress. (C) 2020 Elsevier Ltd. All rights reserved.