A Cell-type-resolved Liver Proteome.
A Cell-type-resolved Liver Proteome.
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DOI：
10.1074/mcp.m116.060145
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发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
He F
中科院分区：
文献类型：
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作者：
Ding C;Li Y;Guo F;Jiang Y;Ying W;Li D;Yang D;Xia X;Liu W;Zhao Y;He Y;Li X;Sun W;Liu Q;Song L;Zhen B;Zhang P;Qian X;Qin J;He F
实质器官由多种细胞类型组成，主要定义为实质细胞（PC）和非实质细胞（NPC）。这些器官的细胞特征还不清楚。蛋白质组学研究有助于解析器官中不同细胞类型的分子细节。这些研究大大扩展了我们对器官发生和器官细胞组成的认识。在这里，我们提出了一个细胞类型分辨肝脏蛋白质组图谱。深入的蛋白质组学为每种细胞类型确定了6000至8000个基因产物（GP），四种细胞类型总共确定了10，075个GP。该数据集揭示了肝脏细胞组成的特征：（1）肝细胞（PC）表达最少的GP，具有独特但高度同质的蛋白质组模式，并执行基本的肝脏功能：（2）PC和NPC之间的劳动分工遵循PC是途径的主要组成部分，但NPC触发途径的模型;以及（3）NPC和PC之间的串扰维持PC表型。本研究以细胞分辨率呈现肝脏蛋白质组，作为在分子水平上解剖细胞类型构成和器官特征的研究模型。
Parenchymatous organs consist of multiple cell types, primarily defined as parenchymal cells (PCs) and nonparenchymal cells (NPCs). The cellular characteristics of these organs are not well understood. Proteomic studies facilitate the resolution of the molecular details of different cell types in organs. These studies have significantly extended our knowledge about organogenesis and organ cellular composition. Here, we present an atlas of the cell-type-resolved liver proteome. In-depth proteomics identified 6000 to 8000 gene products (GPs) for each cell type and a total of 10,075 GPs for four cell types. This data set revealed features of the cellular composition of the liver: (1) hepatocytes (PCs) express the least GPs, have a unique but highly homogenous proteome pattern, and execute fundamental liver functions; (2) the division of labor among PCs and NPCs follows a model in which PCs make the main components of pathways, but NPCs trigger the pathways; and (3) crosstalk among NPCs and PCs maintains the PC phenotype. This study presents the liver proteome at cell resolution, serving as a research model for dissecting the cell type constitution and organ features at the molecular level.