Injectable silk fibroin peptide nanofiber hydrogel composite scaffolds for cartilage regeneration
Injectable silk fibroin peptide nanofiber hydrogel composite scaffolds for cartilage regeneration
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DOI：
10.1016/j.mtbio.2024.100962
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发表时间：
2024-01
影响因子：
8.2
通讯作者：
Deguang Wu;Jian Li;Chengxinqiao Wang;Zhiwen Su;Hao Su;Yan Chen;Bo Yu
中科院分区：
文献类型：
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作者：
Deguang Wu;Jian Li;Chengxinqiao Wang;Zhiwen Su;Hao Su;Yan Chen;Bo Yu
转化生长因子-β1（TGF-β1）对于软骨再生至关重要，但其对酶变性的敏感性和高成本限制了其应用。在此，我们报道了 Ac-LIANAKGFEFEFKFK-NH2(LKP)，一种可以模仿 TGF-β1 功能的自组装肽纳米纤维水凝胶。 LKP水凝胶合成简单，体外实验证实其良好的生物相容性和软骨促进能力。然而，LKP水凝胶的机械性能较差，且容易碎裂；因此，我们通过将LKP与甲基丙烯酸缩水甘油酯（GMA）修饰的丝素蛋白（SF）结合制备了一系列可注射水凝胶复合支架（SF-GMA/LKP）。 SF-GMA/LKP复合支架瞬间诱导软骨缺损的原位填充，同时依靠LKP和SF-GMA之间的相互作用来延长LKP的作用持续时间。 SF-GMA/LKP10和SF-GMA/LKP20复合支架对新软骨和软骨下骨重建效果最好。这种复合水凝胶支架可用于高质量的软骨修复。
Transforming growth factor-β1 (TGF-β1) is essential for cartilage regeneration, but its susceptibility to enzymatic denaturation and high cost limit its application. Herein, we report Ac-LIANAKGFEFEFKFK-NH2(LKP), a self-assembled peptide nanofiber hydrogel that can mimic the function of TGF-β1. The LKP hydrogel is simple to synthesize, andin vitroexperiments confirmed its good biocompatibility and cartilage-promoting ability. However, LKP hydrogels suffer from poor mechanical properties and are prone to fragmentation; therefore, we prepared a series of injectable hydrogel composite scaffolds (SF-GMA/LKP) by combining LKP with glycidyl methacrylate (GMA)-modified silk fibroin (SF). SF-GMA/LKP composite scaffolds instantaneously inducedin-situfilling of cartilage defects and, at the same time, relied on the interaction between LKP and SF-GMA interaction to prolong the duration of action of LKP. The SF-GMA/LKP10 and SF-GMA/LKP20 composite scaffolds had the best effect on neocartilage and subchondral bone reconstruction. This composite hydrogel scaffold can be used for high-quality cartilage repair.