Contributions of P2- and P22-like prophages to understanding the enormous diversity and abundance of tailed bacteriophages.
Contributions of P2- and P22-like prophages to understanding the enormous diversity and abundance of tailed bacteriophages.
复制标题
DOI：
10.1016/j.virol.2016.05.022
复制
发表时间：
2016-09
期刊：
影响因子：
3.7
通讯作者：
Grose JH
中科院分区：
文献类型：
--
作者：
Casjens SR;Grose JH
我们在已报道的沙门氏菌属3298个细菌的全基因组序列中鉴定了20个已知类型的9371个尾噬菌体。其中包括4758个P2型和744个P22型前驱噬菌体。在127个和24个细菌寄主属的基因组序列中发现了后一种噬菌体类型，使已知的噬菌体寄主范围分别增加了114和20个属。这些噬菌体的核苷酸序列比之前已知的48个P2和24个P22型正宗噬菌体的核苷酸序列显示出更多的多样性，这些噬菌体的基因组已经被测序。对这些噬菌体序列的更详细分析表明，尾噬菌体簇或类型之间的主要衣壳蛋白(MCP)基因交换极其罕见，P22原噬菌体编码的尾刺与宿主表面的多糖结构完美对应；因此，MCP和尾穗序列分别准确地预测尾噬菌体类型(从而生活方式)和宿主细胞表面多糖结构。
We identified 9371 tailed phage prophages of 20 known types in reported complete genome sequences of 3298 bacteria in the Salmonella genus. These include 4758 P2 type and 744 P22 type prophages. The latter prophage types were found in the genome sequences of 127 and 24 bacterial host genera, increasing the known host ranges of phages in these groups by 114 and 20 genera, respectively. These prophage nucleotide sequences displayed much more diversity than was previously known from the 48 P2 and 24 P22 type authentic phages whose genomes have been sequenced. More detailed analysis of these prophage sequences indicated that major capsid protein (MCP) gene exchange between tailed phage clusters or types is extremely rare and that P22 prophage-encoded tailspikes correspond perfectly with their hosts’ surface polysaccharide structure; thus, MCP and tailspike sequences accurately predict tailed phage type (and thus lifestyle) and host cell surface polysaccharide structure, respectively.