Healing characteristics of electrospun polyurethane grafts with various porosities
Healing characteristics of electrospun polyurethane grafts with various porosities
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DOI：
10.1016/j.actbio.2012.12.009
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发表时间：
2013-04-01
影响因子：
9.7
通讯作者：
Schima, Heinrich
中科院分区：
文献类型：
--
作者：
Bergmeister, Helga;Schreiber, Catharina;Schima, Heinrich
孔径和孔隙率控制着电纺血管织物中细胞迁移的速率和深度，因此对长期移植成功有很大影响。在这项研究中，我们研究了移植物孔隙率对体外和体内细胞迁移的影响。聚氨酯（PU）接枝物通过静电纺丝制备为细目、低孔隙率接枝物（空隙率（VF）53%）和粗目、高孔隙率接枝物（VF 80%）。在体外评估所制造的移植物的内皮细胞附着和增殖。在大鼠模型中对假体进行为期 7 天、1、3 或 6 个月（每个时间点 n = 7）的研究，并在回收后通过生物力学分析和各种组织学技术进行分析。通过计算机辅助形态测定法计算细胞迁移。在体外，细孔网有利于早期细胞附着。在体内，粗网状移植物显示在导管壁的所有区域的所有时间点上显着更高的细胞群。生物力学测试表明植入前后具有足够的顺应性、拉伸强度和缝合线保留强度。增加的孔隙率可改善宿主细胞在电纺导管中的向内生长和存活。这些导管显示出成功的自然宿主血管重建，且不限制生物力学特性。 (C) 2012 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Pore size and porosity control the rate and depth of cellular migration in electrospun vascular fabrics and thus have a strong impact on long-term graft success. In this study we investigated the effect of graft porosity on cell migration in vitro and in vivo. Polyurethane (PU) grafts were fabricated by electrospinning as fine-mesh, low-porosity grafts (void fraction (VF) 53%) and coarse-mesh, high-porosity grafts (VF 80%). The fabricated grafts were evaluated in vitro for endothelial cell attachment and proliferation. Prostheses were investigated in a rat model for either 7 days, 1, 3 or 6 months (n = 7 per time point) and analyzed after retrieval by biomechanical analysis and various histological techniques. Cell migration was calculated by computer-assisted morphometry. In vitro, fine-pore mesh favored early cell attachment. In vivo, coarse mesh grafts revealed significantly higher cell populations at all time points in all areas of the conduit wall. Biomechanical tests indicated sufficient compliance, tensile and suture retention strength before and after implantation. Increased porosity improves host cell ingrowth and survival in electrospun conduits. These conduits show successful natural host vessel reconstitution without limitation of biomechanical properties. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.