Skeletal muscle proteomics: carbohydrate metabolism oscillates with seasonal and torpor-arousal physiology of hibernation
Skeletal muscle proteomics: carbohydrate metabolism oscillates with seasonal and torpor-arousal physiology of hibernation
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DOI：
10.1152/ajpregu.00298.2011
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发表时间：
2011-11-01
影响因子：
2.8
通讯作者：
Martin, Sandra L.
中科院分区：
文献类型：
--
作者：
Hindle, Allyson G.;Karimpour-Fard, Anis;Martin, Sandra L.
首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细内容骨骼肌蛋白质组学：碳水化合物代谢随着冬眠的季节性和麻木唤醒生理学而振荡。Am J Physiol Regul Integr Comp Physiol 301：R1440-R1452,2011.2011年8月24日首次出版；doi：10.1152/ajpregu.00298.2011。-小型哺乳动物冬眠的生理学在一年多的时间里发生了深刻的变化，从夏季恒温到冬季异温。麻木-觉醒周期定义了冬季高幅度的组织活动波动，特别是骨骼肌，这有助于通过颤抖来进行高能量要求的复温过程。为了更好地了解夏季-冬季和麻木-觉醒转换背后的生物化学，我们应用二维凝胶电泳联用液相色谱/质谱学/质谱仪对13只黄鼠(Ictidomys Tridecemlineatus)在两个夏季和六个冬季状态下的后肢肌肉中的可溶性蛋白质进行了分析。216个蛋白质斑点因采样状态不同而不同。值得注意的是，尽管冬季各州的肌肉活动水平存在很大差异，但冬季内蛋白质调节是数据集的一个次要组成部分；相反，大部分差异(127/138个明确识别的蛋白质点)发生在夏季和冬季之间。我们没有发现这种冬眠者骨骼肌萎缩的任何蛋白质组学特征，也没有发现蛋白质代谢的任何不同的季节性调节。相反，对代谢底物偏好的调整主导了检测到的蛋白质组差异。碳水化合物代谢途径(糖酵解和糖异生)夏季丰富，而冬季蛋白质组脂肪酸β-氧化丰富。然而，我们的数据表明，在冬季，人们对碳水化合物储备的依赖程度保持不变。磷酸葡萄糖变位酶(PGM1)，可逆地为糖酵解或糖生成准备葡萄糖亚基，显示出明显的冬季状态特异性磷酸化。PGM1在复温过程中被磷酸化，并被间歇唤醒去磷酸化，这意味着在复温过程中葡萄糖补充了脂类燃料。这一点，加上冬季TCA循环酶的升高，表明后肢肌肉已经为快速产生能量做好了准备，碳水化合物是发抖产热的重要燃料。
Hindle AG, Karimpour-Fard A, Epperson LE, Hunter LE, Martin SL. Skeletal muscle proteomics: carbohydrate metabolism oscillates with seasonal and torpor-arousal physiology of hibernation. Am J Physiol Regul Integr Comp Physiol 301: R1440-R1452, 2011. First published August 24, 2011; doi:10.1152/ajpregu.00298.2011.-The physiology of small mammalian hibernators shifts profoundly over a year, from summer homeothermy to winter heterothermy. Torpor-arousal cycles define high-amplitude tissue activity fluctuations in winter, particularly for skeletal muscle, which contributes to the energetically demanding rewarming process via shivering. To better understand the biochemistry underlying summer-winter and torpor-arousal transitions, we applied two-dimensional gel electrophoresis coupled with liquid chromatography/mass spectrometry/mas spectrometry to the soluble proteins from hindlimb muscle of 13-lined ground squirrels (Ictidomys tridecemlineatus) in two summer and six winter states. Two hundred sixteen protein spots differed by sampled state. Significantly, intrawinter protein adjustment was a minor component of the dataset despite large discrepancies in muscle activity level among winter states; rather, the bulk of differences (127/138 unequivocally identified proteins spots) occurred between summer and winter. We did not detect any proteomic signatures of skeletal muscle atrophy in this hibernator nor any differential seasonal regulation of protein metabolism. Instead, adjustments to metabolic substrate preferences dominated the detected proteomic differences. Pathways of carbohydrate metabolism (glycolysis and gluconeogenesis) were summer enriched, whereas the winter proteome was enriched for fatty acid beta-oxidation. Nevertheless, our data suggest that some reliance on carbohydrate reserves is maintained during winter. Phosphoglucomutase (PGM1), which reversibly prepares glucose subunits for either glycolysis or glycogenesis, showed apparent winter state-specific phosphorylation. PGM1 was phosphorylated during rewarming and dephosphorylated by interbout arousal, implying that glucose supplements lipid fuels during rewarming. This, along with winter elevation of TCA cycle enzymes, suggests that hindlimb muscles are primed for rapid energy production and that carbohydrates are an important fuel for shivering thermogenesis.