An experimental study on the effects of ethanol and folic acid deficiency, alone or in combination, on pregnant Swiss mice
An experimental study on the effects of ethanol and folic acid deficiency, alone or in combination, on pregnant Swiss mice
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DOI：
10.1080/00313020701449290
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发表时间：
2007-01-01
期刊：
影响因子：
4.5
通讯作者：
Peres, Luiz Cesar
中科院分区：
文献类型：
--
作者：
Gutierrez, Cristiane Minot;de Morais Ribeiro, Camila Nunes;Peres, Luiz Cesar
简介：乙醇具有致畸性，会干扰叶酸，被年轻女性广泛使用。我们的目的是确定乙醇和/或叶酸缺乏对小鼠胎儿的影响。方法：在实验1中，接受商业饮食的怀孕小鼠被分为三组：对照组（C）、低乙醇剂量组（LE，0.4 g/kg）和高乙醇剂量组（HE，4.0 g/kg）。在实验2中，接受无叶酸饮食（FFD）的怀孕小鼠被分为三组：叶酸缺乏（FD）、叶酸缺乏加低剂量乙醇（FDLE）和叶酸缺乏加高乙醇剂量（FDHE）。 C组和FD组接受盐水，其余组接受静脉注射乙醇。 p。妊娠第7天至第9天（GD），第18个GD处死。结果：实验1中，HE组比C组和LE组出现先天性异常、晚期胎死（LFD）、胎儿身长和体重以及胎盘重量和直径较低。在实验2中，与FD组相比，FDLE组和FDHE组的活胎数量较少，重吸收和LFD数量较多，身长较小，胎儿体重、胎盘重量和直径较低。结论：在接受商业饮食的动物中，高剂量乙醇对妊娠有害，会诱发先天性异常、宫内生长受限、胎盘缩小和LFD增加，这些事件在低剂量时不会发生。然而，在无叶酸饮食中，低剂量的乙醇与高剂量的乙醇一样有害。
Introduction: Ethanol is teratogenic, interferes with folic acid and is extensively used by young women. Our objective was to determine the effects of ethanol and/or folate deficiency on mouse fetuses.Method: In Experiment 1, pregnant mice receiving a commercial diet were divided into three groups: control (C), low ethanol dose (LE, 0.4 g/kg), and high ethanol dose (HE, 4.0 g/kg). In Experiment 2, pregnant mice receiving a folatefree diet (FFD) were divided into three groups: folate deficiency (FD), folate deficiency plus a low ethanol dose (FDLE), and folate deficiency plus a high ethanol dose (FDHE). Groups C and FD received saline and the remaining groups received ethanol administered i. p. from the 7th to the 9th gestational day (GD) and were sacrificed on the 18th GD.Results: In Experiment 1, Group HE presented congenital anomalies, late fetal death (LFD), lower fetal length and weight and placental weight and diameter than Groups C and LE. In Experiment 2, there was a smaller number of live fetuses, a larger number of reabsorptions and LFD, a smaller length and lower fetal weight, placental weight and diameter in Groups FDLE and FDHE than in Group FD.Conclusion: In animals receiving a commercial diet, a high ethanol dose is deleterious to the pregnancy, inducing congenital anomalies, intrauterine growth restriction, reduction of the placenta and increased LFD, events that did not occur with the low dose. However, with a folate free diet, a low ethanol dose is as deleterious as a high dose.