Expression of the JAK/STAT3/SOCS3 signaling pathway in herniated lumbar discs
Expression of the JAK/STAT3/SOCS3 signaling pathway in herniated lumbar discs
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DOI：
10.1016/j.neulet.2014.03.045
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发表时间：
2014-05-21
影响因子：
2.5
通讯作者：
Takayasu, Masakazu
中科院分区：
文献类型：
--
作者：
Osuka, Koji;Usuda, Nobuteru;Takayasu, Masakazu
炎症细胞因子白细胞介素6（IL-6）在引起腰椎间盘突出症症状中起重要作用。本研究阐明了IL-6信号通路在椎间盘突出中的表达。对 10 名患者的腰椎间盘突出制备的匀浆进行了评估。通过蛋白质印迹分析检查 janus 激酶 1 (JAK1)、信号转导子和转录激活子 3 (STAT3)、Tyr(705) 磷酸化 (p)-STAT3、细胞因子信号传导抑制子 3 (SOCS3) 和肌动蛋白的表达。还通过免疫染色检查了 Tyr(705) 处 JAK1、STAT3 和 p-STAT3 的表达。几乎所有病例中均检测到 JAK1、STAT3、Tyr(705) 处的 p-STAT3 和 SOCS3。针对JAK1和STAT3的免疫反应性主要在软骨细胞中观察到，而针对Tyr(705)的p-STAT3的免疫反应性在软骨细胞的细胞核中观察到。 JAK/STAT信号通路可能被IL-6激活并将信息从细胞表面传递到细胞核，并且该通路受到SOCS3的负调控。这些 JAK1、STAT3 和 SOCS3 分子可能严格调节椎间盘突出的软骨细胞的退化并在其中发挥作用。 (C) 2014 Elsevier Ireland Ltd. 保留所有权利。
The inflammatory cytokine interleukin-6 (IL-6) plays an important role in causing symptoms of lumbar disk herniation. The present study clarifies the expression of the signaling pathway of IL-6 in herniated discs. Homogenates prepared from lumbar herniated discs from 10 patients were assessed. The expression of janus kinase 1 (JAK1), signal transducer and activator of transcription 3 (STAT3), phosphorylated (p)-STAT3 at Tyr(705), suppressor of cytokine signaling 3 (SOCS3) and actin was examined by Western blot analysis. The expression of JAK1, STAT3, and p-STAT3 at Tyr(705) was also examined by immunostaining. JAK1, STAT3, p-STAT3 at Tyr(705) and SOCS3 were detected in almost all cases. Immunoreactivity against JAK1 and STAT3 was observed mainly in chondrocytes, whereas immunoreactivity against p-STAT3 at Tyr(705) was observed in the nuclei of chondrocytes. The JAK/STAT signaling pathway might be activated by IL-6 and transmit messages from the cell surface to the nucleus, and the pathway is negatively regulated by SOCS3. These JAK1, STAT3 and SOCS3 molecules might tightly regulate and play a role in the degeneration of chondrocytes within herniated discs. (C) 2014 Elsevier Ireland Ltd. All rights reserved.