Impaired phagocytosis of nontypeable Haemophilus influenzae by human alveolar macrophages in chronic obstructive pulmonary disease
Impaired phagocytosis of nontypeable Haemophilus influenzae by human alveolar macrophages in chronic obstructive pulmonary disease
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DOI：
10.1086/508428
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发表时间：
2006-11-15
影响因子：
6.4
通讯作者：
Sethi, Sanjay
中科院分区：
文献类型：
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作者：
Berenson, Charles S.;Garlipp, Mary Alice;Sethi, Sanjay
背景慢性阻塞性肺疾病（COPD）中不可分型流感嗜血杆菌（NTHI）与人肺泡巨噬细胞的相互作用与NTHI的持续存在有关。然而，介导NTHI诱导的巨噬细胞反应的免疫机制知之甚少。我们假设COPD患者肺泡巨噬细胞对NTHI的免疫应答受损。将从患有COPD的戒烟者（n=14）、没有COPD的戒烟者（n=15）和非吸烟者（n=9）获得的血液和肺泡巨噬细胞与从患有COPD的患者获得的3种不同的NTHI菌株一起孵育。测定H-3-NTHI的吞噬作用（表示为平均总放射性的百分比）和细胞内活力（评估为活细胞相关NTHI的百分比）。与非COPD供体相比，COPD供体的肺泡巨噬细胞吞噬功能受损（每种NTHI菌株的中位数[四分位距]）：14 P13 H5，0.26 6P5H1，0.92（0.32-1.82）vs1.90（1.32 -2.68）; 14P14H1，0.79（0.23-1.32）vs2.13（1.13-2.40）（P
Background. Interactions of nontypeable Haemophilus influenzae (NTHI) with human alveolar macrophages are implicated in the persistence of NTHI in chronic obstructive pulmonary disease (COPD). However, the immunologic mechanisms that mediate NTHI-induced macrophage responses are poorly understood. We hypothesized that immunologic responses of alveolar macrophages to NTHI are impaired in COPD.Methods. Blood and alveolar macrophages - obtained from ex-smokers with COPD (n=14), ex-smokers without COPD (n=15), and nonsmokers (n=9) - were incubated with 3 distinct NTHI strains obtained from patients with COPD. Phagocytosis of H-3-NTHI, expressed as a percentage of the mean total radioactivity, and of intracellular viability, assessed as a percentage of viable cell-associated NTHI, were measured.Results. Alveolar macrophages from donors with COPD, compared with those from donors without COPD, had impaired phagocytosis (median [interquartile range]) for each NTHI strain: 14P13H5, 0.26 (0.08-0.61) versus 1.36 (0.69-1.95); 6P5H1, 0.92 (0.32-1.82) versus 1.90 (1.32-2.68); and 14P14H1, 0.79 (0.23-1.32) versus 2.13 (1.13-2.40) (P